alexa Effects of Irrigation Regimes on Antioxidant Activity and Total Phenolic Content of Thyme (Thymus vulgaris L.)
ISSN: 2167-0412

Medicinal & Aromatic Plants
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Research Article

Effects of Irrigation Regimes on Antioxidant Activity and Total Phenolic Content of Thyme (Thymus vulgaris L.)

Khosh-Khui M*, Ashiri F and Saharkhiz MJ
Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran
Corresponding Author : Khosh-Khui M
Department of Horticultural Science
College of Agriculture
Shiraz University
Shiraz, Iran
Received October 08, 2012; Accepted October 10, 2012; Published October 17, 2012
Citation: Khosh-Khui M, Ashiri F, Saharkhiz MJ (2012) Effects of Irrigation Regimes on Antioxidant Activity and Total Phenolic Content of Thyme (Thymus vulgaris L.). Med Aromat Plants 1:114. doi: 10.4172/2167-0412.1000114
Copyright: © 2012 Khosh-Khui M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Drought is a major abiotic factor that limits agricultural crop production. Antioxidant activity of some plants may be changed by water stress. In this study, a pot experiment was conducted to assess the effect of drought on the antioxidant activity of thyme (Thymus vulgaris L.). Different irrigation regimes (2, 4, 6, 8 and 10 day intervals) were applied for about 6 months and the antioxidant capacity was evaluated by using two different assays; the FRAP (ferric reducing antioxidant power) and DPPH (2,2-diphenylpicrylhydrazyl) scavenging assays. Although in some other species water stress might increase the antioxidant capacity, but the experimental results showed that long-term drought caused
a reduction in the antioxidant activity and total phenolic content of thyme. While all plants died with 10 day irrigation interval, with 2 (control), 4, 6, and 8 days irrigation intervals, IC50 values of the DPPH assay was 87.82, 105.86, 118.05 and 133.60 μg/ml respectively. A higher value of IC50 showed a lower antioxidant activity, which indicated that severe water stress (8 days) significantly decreased the antioxidant activity of thyme (P≤0.05). FRAP values showed a trend to reduction by increasing the irrigation intervals, but this trend was not significant.


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