Effects of Lipofundin ÃÂ® on the Measurement of Total Bilirubin by SpectrophotometryJu-Ting Hsueh 1 , Kwei-Shuai Hwang2 , Lu Pai3 , Yu-Lueng Shih4 , Jen-Jiuan Liaw5 , Shyi-Jou Chen6 , Shinn-Ying Ho7 and Hueng-Chuen Fan6 *
- *Corresponding Author:
- Hueng-Chuen Fan, MD, PhD
Department of Pediatrics, Tri-Service General Hospital
National Defense Medical Center, No 325, Sec 2
Chenggong Rd, Neihu Dist, Taipei City 114, Taiwan
Tel: +886 2 87923311-13628
Fax: +886 2 87927293
E-mail: [email protected]
Received date: October 05, 2011; Accepted date: November 22, 2011; Published date: November 25, 2011
Citation: Hsueh JT, Hwang KS, Pai L, Shih YL, Liaw JJ, Chen SJ, et al. (2011) Effects of Lipofundin® on the Measurement of Total Bilirubin by Spectrophotometry. J Steroids Horm Sci 4:118. doi:10.4172/2157-7536.1000118
Copyright: © 2011 Hsueh JT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Jaundice occurs frequently in neonates and can cause severe neurological complications; hence, hyperbilirubinemia is usually monitored by direct spectrophotometry. However, lipemia, resulting from inborn disorders or parenteral feeding of preterm neonates with lipid emulsion, may interfere with certain laboratory assessments. Here, we evaluated whether artificial lipemia also interferes with bilirubin measurement by direct spectrophotometry. Methods: Total bilirubin levels were assessed by the spectrophotometry when serial concentrations of Lipofundin®, medium-chain triglycerides, or a stabilizer solution, were added to cord blood samples from five full-term and five preterm newborn infants. Results: In blood specimens from ten neonates, spectrophotometry-determined bilirubin levels proportionally and significantly increased in the presence of Lipofundin® at least 1% v/v or 10% medium-chain triglycerides at least 10% v/v in all pre-term and full-term infants. The stabilizer solution caused no interference. Conclusion: Lipofundin® in the cord blood interferes with spectrophotometric measurement of total bilirubin; this effect is mainly related to triglyceride levels and has implications for management of neonates with jaundice.