alexa Effects of Obesity and 7,12-Dimethylbenz(A) Anthracene (DMBA) Treatment on Liver Cytochrome P4501A1 and 1B1 Expression in Ovariectomized Obese Zucker Rats
ISSN: 1747-0862

Journal of Molecular and Genetic Medicine
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Research Article

Effects of Obesity and 7,12-Dimethylbenz(A) Anthracene (DMBA) Treatment on Liver Cytochrome P4501A1 and 1B1 Expression in Ovariectomized Obese Zucker Rats

Reza Hakkak1*,2,3, Stewart Macleod2,3 and Soheila Korourian4

1Departments of Dietetics and Nutrition, University of Arkansas for Medical Sciences, 4301 W. Markham St., Little Rock, AR 72205, USA

2Departments of Pediatrics, University of Arkansas for Medical Sciences, 4301 W. Markham St., Little Rock, AR 72205, USA

3Arkansas Children’s Hospital Research Institute, 13 Children’s Way. Little Rock, AR 72202, USA

4Department of Pathology, University of Arkansas for Medical Sciences, 4301 W. Markham St., Little Rock, AR 72205, USA

Corresponding Author:
Dr. Reza Hakkak
Department of Dietetics and Nutrition 4301 W. Markham St., Mail Slot 627
Little Rock, AR 72205, USA
Tel: 501-686-6166
Fax: 501-686-5716
E-mail: [email protected]

Received Date: October 07, 2013; Accepted Date: October 30, 2013; Published Date: November 06, 2013

Citation: Hakkak R, Macleod S, Korourian S (2013) Effects of Obesity and 7,12-Dimethylbenz(A) Anthracene (DMBA) Treatment on Liver Cytochrome P4501A1 and 1B1 Expression in Ovariectomized Obese Zucker Rats. J Mol Genet Med S1:003. doi:10.4172/1747-0862.S1-003

Copyright: © 2013 Hakkak R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

Obesity is a risk factor for postmenopausal breast cancer development. We have shown that obesity increases DMBA-induced mammary tumor development in intact and ovariectomized Zucker rats. Several data suggest that DMBA requires metabolic activation to exert its carcinogenic effects. The objective of this study was to investigate the effect of obesity on hepatic expression of cytochrome P450 CYP1A1 and CYP1B1 following DMBA treatment in obese and lean ovariectomized Zucker rats. Forty day-old, ovariectomized obese (n=20) and lean (n=20) Zucker rats were placed on AIN-93 G diet and 10 days later were orally gavaged with either with sesame oil (control) or with 65 mg/kg DMBA in sesame oil. All rats were sacrificed 24 hours post-DMBA treatment. Liver microsomes were prepared, and CYP1A1 and CYP1B1 expression was measured by Western blotting using goat anti-mouse CYP1A1 and CYP1B1 antibodies. DMBA treatment significantly (p<0.001) increased expression of CYP1A1 in lean and obese ovariectomized rats compared to the control group. CYP1B1 expression was not affected by obesity or DMBA. These data suggest that DMBA can increase the expression of enzymes that are responsible for DMBA metabolism in the ovariectomized Zucker rat model.

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