Effects of Polylysine and Polyglutamate on Inflammation and the Normal Process of Peritoneal Healing After Surgery
Daniel Åkerberg, Karolin Isaksson, Monica Posaric-Bauden, Roland Andersson and Bobby Tingstedt*
Department of Surgery, Clinical Sciences, Lund University, Lund, Sweden
- Corresponding Author:
- Bobby Tingstedt, MD, Ph.D
Associate Professor, Institution for Clinical Sciences
Lund University, Departement of Surgery
University Hospital of Skåne at Lund
Getingevägen 4221 85 Lund, Sweden
E-mail: [email protected]
Received date: May 10, 2012; Accepted date: May 28, 2012; Published date: May 30, 2012
Citation: Åkerberg D, Isaksson K, Posaric-Bauden M, Andersson R, Tingstedt B (2012) Effects of Polylysine and Polyglutamate on Inflammation and the Normal Process of Peritoneal Healing After Surgery. J Tissue Sci Eng 3:117. doi:10.4172/2157-7552.1000117
Copyright: © 2012 Åkerberg D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Introduction: Intraperitoneal adhesions are common after abdominal surgery and may lead to serious clinical complications. Previous studies have investigated the possible effects of the polypeptides poly-L-lysine (aPL) and poly-L-glutamate (PG) forming a polymer complex that prohibits local peritoneal adhesions after surgery. The aim of this study was to examine whether the normal process of peritoneal healing was affected by PL/PG polymer matrix.
Material and methods: Male rats (Sprague Dawley) (n=84) underwent abdominal wall surgery and suturing. Rats were randomized in groups according to evaluation time (2, 4, 6, 8, 24 hours and 7 days) with corresponding control groups. Controls received saline (0.9%) and the experimental groups received PL/PG on the surgery site. tPA, PAI-1, IL-6 and active TGFb1 were analyzed at given time points postoperatively in peritoneal lavage. Adhesions were evaluated after seven days. Significant differences were considered to be p<0.05.
Results: At a few individual time points small differences were seen between the groups (control and experiment)
comparing levels of tPA, PAI-1, IL-6 and active TGFb1. When comparing levels of substances from all time points no statistical differences were seen between the groups as a total. Adhesions were significantly decreased on day 7, p=0.002.
Conclusion: Despite significant reduction in adhesions PL/PG administered intraperitoneally as an anti-adhesion agent locally on surgically traumatized area does not seem to affect the normal process of peritoneal healing.