alexa Effects of Prenatal Testosterone Exposure on Sexually D
ISSN: 2157-7536

Journal of Steroids & Hormonal Science
Open Access

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Research Article

Effects of Prenatal Testosterone Exposure on Sexually Dimorphic Gene Expression in the Neonatal Mouse Cortex and Hippocampus

Chris Armoskus, Thomas Mota, Debbie Moreira and Houng-Wei Tsai*

Department of Biological Sciences, California State University, Long Beach, CA 90840, USA

*Corresponding Author:
Houng-Wei Tsai
Department of Biological Sciences
California State University Long Beach
1250 Bellflower Boulevard, Long Beach
CA 90840-3702, USA
Tel: (562) 985-8878
E-mail: [email protected]

Received date: May 12, 2014; Accepted date: July 16, 2014; Published date: July 23, 2014

Citation: Armoskus C, Mota T, Moreira D, Tsai HW (2014) Effects of Prenatal Testosterone Exposure on Sexually Dimorphic Gene Expression in the Neonatal Mouse Cortex and Hippocampus. J Steroids Hormon Sci 5:139 doi:10.4172/2157-7536.1000139

Copyright: © 2014 Armoskus C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Using gene expression microarrays and reverse transcription with quantitative polymerase chain reaction (RT-qPCR), we have recently identified several novel genes that are differentially expressed in the neonatal male versus female mouse cortex/hippocampus (Armoskus et al.). Since perinatal testosterone (T) secreted by the developing testes masculinizes cortical and hippocampal structures and the behaviors regulated by these brain regions, we hypothesized that sexually dimorphic expression of specific selected genes in these areas might be regulated by T during early development.

To test our hypothesis, we treated timed pregnant female mice daily with vehicle or testosterone propionate (TP) starting on embryonic day 16 until the day of birth. The cortex/hippocampus was collected from vehicle- and TP-treated, male and female neonatal pups. Total RNA was extracted from these brain tissues, followed by RT-qPCR to measure relative mRNA levels of seven sex chromosome genes and three autosomal genes that have previously showed sex differences.

The effect of prenatal TP was confirmed as it stimulated Dhcr24 expression in the neonatal mouse cortex/hippocampus and increased the anogenital distance in females. We found a significant effect of sex, but not TP, on expression of three Y-linked (Ddx3y, Eif2s3y, and Kdm5d), four X-linked (Eif2s3x, Kdm6a, Mid1, and Xist), and one autosomal (Klk8) genes in the neonatal mouse cortex/hippocampus.

Although most of the selected genes are not directly regulated by prenatal T, their sexually dimorphic expression might play an important role in the control of sexually differentiated cognitive and social behaviors as well as in the etiology of sex-biased neurological disorders and mental illnesses.


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