Effects of Rifampicin on Experimental Spinal Cord Ischemia/Reperfusion Injury in Rats
|Erhan Arslan1*, Ertugrul Cakir2 and Murat Selcuk Eminagaoglu3|
|1Department of Neurosurgery, School of Medicine, Giresun University, Giresun, Turkey|
|2Department of Neurosurgery, School of Medicine, Karadeniz Technical University, Trabzon, Turkey|
|3Department of Biochemistry, Ozel Medline Hospital, PC: 26061, Eskisehir, Turkey|
|Corresponding Author :||Erhan Arslan, MD
Department of Neurosurgery
School of Medicine, Giresun University
Nizamiye Mah. Mumcular Sok. No: 1/1
PC:28000, Merkez/Giresun, Turkey
E-mail: [email protected]
|Received April 11, 2014; Accepted May 23, 2014; Published May 28, 2014|
|Citation: Arslan E, Cakir E and Eminagaoglu MS (2014) Effects of Rifampicin on Experimental Spinal Cord Ischemia/Reperfusion Injury in Rats. J Spine 3:167. doi:10.4172/2165-7939.1000167|
|Copyright: © 2014 Arslan E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Objective: The effect of rifampisin was examined using a spinal cord ischemia/reperfusion (I/R) injury model in rats.
Materials and Methods: 25 Wistar Albino rats weighing 200-250 g were used for the study. Rats were divided in 5 groups. After laparatomy, aorta was clamped 45 minutes below the left renal artery in all groups except shamoperated group. 1 cc saline was injected to vehicle group and rifampicin (20 mg/kg) was administered to treatment group intraperitoneally. In group 5, 20 mg/kg of rifampicin applied intraperitoneally before laparotomy. 2 hours after application of rifampicin, the animals underwent clip compression for 45 minutes after exposure of the abdominal aorta. At 1 h and 24 h, all groups were examined for neurologic outcome according to Tarlov scale. At 24 h, rats were sacrificed. The spinal cord was excised by laminectomy between the T8-12 levels and tissue MDA levels were studied.
Results: At 1 h, difference between motor scores of sham-operated group and other groups was statistically significant (P=0.008). At 24 h, difference between trauma and treatment or p-treatment group was statistically significant (P<0.05). When MDA levels of the groups were compared by using Kruskal Wallis variance analysis, the result was statistically significant (P=0.001). When trauma and vehicle group were compared with treatment group by Mann Whitney U test, the results were statistically significant (P=0.008).
Conclusions: To our knowledge, this is the first study that shows the effects of rifampicin on spinal cord ischemia/reperfusion injury. Rifampicin was found to be effective on spinal cord ischemia/reperfusion injury, but further investigations are mandatory.