alexa Effects of Selected Inhibitors of Protein Kinases and Phosphatases on Cellular Respiration: An In Vitro Study
ISSN: 2161-0495

Journal of Clinical Toxicology
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Research Article

Effects of Selected Inhibitors of Protein Kinases and Phosphatases on Cellular Respiration: An In Vitro Study

Saeeda Almarzooqi1, Alia Albawardi1, Ali S. Alfazari2, Dhanya Saraswathiamma1, Hidaya Mohammed Abdul-Kader2, Sami Shaban3, Robert Mallon4 and Abdul-Kader Souid 5,*

1Departments of Pathology, UAE University, Al-Ain, Abu Dhabi, United Arab Emirates

2Medicine, UAE University, Al-Ain, Abu Dhabi, United Arab Emirates

3Medical Education, UAE University, Al-Ain, Abu Dhabi, United Arab Emirates

4RHP Associates, Caldwell NJ

5Pediatrics, UAE University, Al-Ain, Abu Dhabi, United Arab Emirates

*Corresponding Author:
Abdul-Kader Souid
Departments of Pediatrics, UAE University
Al-Ain, Abu Dhabi, United Arab Emirates
Tel: +9-713-713-7429
Fax: +9-713-767-2022
E-mail: [email protected]

Received date: August 17, 2014; Accepted date: October 1, 2014; Published date: October 4, 2014

Citation: Almarzooqi S, Albawardi A, Alfazari AS, Saraswathiamma D, Abdul-Kader HM, et al. (2014) Effects of Selected Inhibitors of Protein Kinases and Phosphatases on Cellular Respiration: An In Vitro Study. J Clin Toxicol 4:212. doi: 10.4172/2161-0495.1000212

Copyright: © 2014, Souid A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Inhibitors of protein kinases/phosphatases are known to alter cellular metabolism. Effects of these rapidly identified small molecules on cellular respiration (mitochondrial O2 consumption) have not been adequately investigated, especially in healthy organs. This in vitro study measured cellular respiration in tissues from C57BL/6 mice with and without GSK2126458 (PI3K/mTOR inhibitor), BEZ235 (PI3K/mTOR inhibitor), GDC0980 (PI3K/mTOR inhibitor), GSK1120212 (trametinib, MEK inhibitor), sorafenib, regorafenib (multikinase inhibitors), and cyclosporine (calcineurin inhibitor). Cellular respiration was measured by the phosphorescence oxygen analyzer, aided by the O2 probe Pd(II)-meso-tetra-(4-sulfonatophenyl)-tetrabenzoporphyrin. Cyanide inhibited O2 consumption, confirming the oxidation occurred in the respiratory chain. Renal cellular respiration decreased 26-34% in the presence of 10 μM GSK2126458 (p<0.001), 10 μM BEZ235 (p<0.001), or 1.0 μM GDC0980 (p<0.001). Liver cellular respiration decreased 20-32% with 10 μM GSK2126458 (p=0.048), 0.1 μM BEZ235 (p=0.028), or 0.1 μM GDC0980 (p=0.016). Heart cellular respiration decreased 19-27% with 10 μM GSK2126458 (p=0.078), 10 μM BEZ235 (p=0.040), or 10 μM GDC0980 (p=0.036). GSK1120212, sorafenib, regorafenib, and cyclosporine had no effects on cellular respiration. Thus, cellular bioenergetics (the biochemical processes involved in energy conversion) is interconnected with PI3K/PTEN/Akt/mTOR; and inhibitors of this cascade impair cellular respiration. This biomarker (cellular respiration) senses the activity/toxicity of this class of molecularly targeted agents.

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