Elucidation of a "Signal-ON" Mechanism of Aggregation-Induced Emission Dye-Labelled DNA/DNA Duplexes and Application of a Repeat DNA Detection Method
Takashi Murashima*, Koji Kawamura and Ai Matsumoto
Department of Nanobiochemistry, FIRST (Frontiers of Innovative Research in Science and Technology), Konan University, Hyogo, Japan
- *Corresponding Author:
- Takashi Murashima
Department of Nanobiochemistry
FIRST (Frontiers of Innovative Research in Science and Technology)
Konan University, Hyogo 650-0047, Japan
E-mail: [email protected]
Received Date: December 02, 2016; Accepted Date: December 12, 2016; Published Date: December 16, 2016
Citation: Murashima T, Kawamura K, Matsumoto A (2016) Elucidation of a “Signal- ON” Mechanism of Aggregation-Induced Emission Dye-Labelled DNA/DNA Duplexes and Application of a Repeat DNA Detection Method. Med Chem (Los Angeles) 6:704-709. doi: 10.4172/2161-0444.1000418
Copyright: © 2016 Murashima T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
We reveal a fluorescence enhancement mechanism of an aggregation-induced emission (AIE)-DNA probe for target DNA detection. The enhancement is caused by the restriction of intramolecular rotation (RIR) effect that arises from steric hindrance between the AIE dye moiety in a hybridized probe and the dangling end (free-ssDNA) or peripheral dsDNA. Thus, these AIE-DNA probes can selectively detect two or more DNA repeat sequences, such as telomeric repeats.