Endophytic Bacteria from Datura stramonium for Fusarium Wilt Suppression and Tomato Growth Promotion
- *Corresponding Author:
- Aydi Ben Abdallah R
UR13AGR09- Integrated Horticultural
Production in the Tunisian Centre-East
Regional Center of Research on Horticulture and Organic Agriculture
University of Sousse, Tunisia
Tel: +216 73 368 125
E-mail: [email protected]
Received Date: January 06, 2016; Accepted Date: January 20, 2016; Published Date: January 27, 2016
Citation: Ben Abdallah RA, Jabnoun-Khiareddine H, Nefzi A, Mokni-Tlili S, Daami-Remadi M (2016) Endophytic Bacteria from Datura stramonium for Fusarium Wilt Suppression and Tomato Growth Promotion. J Microb Biochem Technol 8:030-041. doi:10.4172/1948-5948.1000259
Copyright: © 2016 Ben Abdallah RA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Ten nonpathogenic bacterial isolates, recovered from Datura stramonium organs, and successfully colonizing the internal stem tissues of tomato cv. Rio Grande were screened for their ability to suppress tomato Fusarium wilt, caused by Fusaium oxysporum f. sp. lycopersici (FOL), and to enhance tomato growth. S37 and S40 isolates were found to be the most effective in decreasing leaf yellowing by 94-88% and the vascular browning extent by 96-95%, respectively, as compared to FOL-inoculated and untreated control. A significant enhancement of growth parameters was recorded on tomato plants inoculated or not with the pathogen. The two bioactive isolates were morphologically and biochemically characterized and identified using 16S rDNA sequencing genes as Stenotrophomonas maltophilia str. S37 and Bacillus mojavensis str. S40. Screened in vitro for their antifungal activity toward FOL, these strains led to 43.8 and 39% decrease in pathogen radial growth and to the formation of an inhibition zone of about 11.37 and 12.12 mm in diameter, respectively. S. maltophilia str. S37 and B. mojavensis str. S40 were found to be chitinase-, protease- and pectinase-producing strains but only S. maltophilia str. S37 was able to produce the volatile metabolite hydrogen cyanide. Indole-3-acetic acid production, phosphate solubilizing ability and pectinase activity were investigated for elucidating their plant growth promoting traits and their endophytic colonization ability. To our knowledge, this is the first report on endophytic bacteria from D. stramonium exhibiting Fusarium wilt suppression potential and plant growth-promoting ability on tomato.