alexa Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

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Research Article

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

Jaroslav Chlupac1,2*, Elena Filova1, Tomas Riedel3, Eduard Brynda3, Elzbieta Pamula4 and Lucie Bacakova1

1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic

2Department of Transplant Surgery, Institute for Clinical and Experimental Medicine, Prague, Czech Republic

3Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic

4Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Krakow, Poland

Corresponding Author:
Jaroslav Chlupac
Department of Biomaterials and Tissue Engineering Institute of Physiology
Academy of Sciences of the Czech Republic
Czech Republic
Tel: +420-296-443-743
Fax: +420-296-442-488
E-mail: [email protected]

Received date: April 21, 2014; Accepted date: June 28 2014; Published date:June 30, 2014

Citation: Chlupac J, Filova E, Riedel T, Brynda E, Pamula E, et al. (2014) Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers. J Tissue Sci Eng 5:139. doi:10.4172/2157-7552.1000139

Copyright: © 2014 Chlupac J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: Bypass surgery for atherosclerosis is confronted with the absence of endothelial cells in the lumen of vascular prosthesis in humans. This imposes a risk of thrombosis. New biomaterials try to minimize surface thrombogenicity.

Methods: Knitted polyethylene terephthalate (PET) vascular graft patches were impregnated with degradable polyester polymers: poly (L-lactide-co-glycolide) (PLG) or poly (L-lactide-co-glycolide-co-ε-caprolactone) (PLGC). The luminal surface was coated with collagen type I (Co) to which extracellular matrix proteins laminin (LM), fibronectin (FN), or surface fibrin gel (Fb) were attached. Three types of prostheses (PET, PET–PLG and PET– PLGC) and 5 types of protein assemblies (+Co, +Co/LM, +Co/FN, +Co/Fb, +Co/Fb/FN) were fabricated. Scanning electron microscopy and measurements of the water contact angles were performed. The development of a bovine endothelial cell layer was studied in a static culture for 1 week.

Results: The cells reached confluence on all PET surfaces with the highest final density on +Co/FN. Impregnation of PET with polymers made it less adhesive for cells in the following order: PET > PET–PLG > PET– PLGC. However, additional coating with the protein assemblies enhanced the endothelial cell growth, especially on fibrin-containing surfaces.

Conclusion: Tri-component vascular grafts composed of PET, copolymers and cell-adhesive assemblies were fabricated. The endothelial lining on the polymer-coated grafts was promoted after modification with the protein multilayers.
Artificial vascular prostheses have been made of non-degradable, non-compliant and thrombogenic materials for more than 50 years. Thus, they resemble passive tubing. Potential bio-activation by degradable materials and by introduction of living endothelial cells may approximate these materials to native artery. This work provided a method to include bio-degradable polymers into vascular graft and to facilitate the growth of cell lining via adhesive protein multilayers.

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