Engineering Escherichia coli to Disrupt Poly-N-Acetylglucosamine Containing Bacterial Biofilms
Vihang Vivek Ghalsasi and Victor Sourjik*
Max Planck Institute of Terrestrial Microbiology and LOEWE Research Center for Synthetic Microbiology (SYNMIKRO), Marburg, Germany
- *Corresponding Author:
- Victor Sourjik
Max Planck Institute of Terrestrial
Microbiology and LOEWE Research Center for Synthetic Microbiology
(SYNMIKRO), Marburg, Germany
Tel: +49 6421 2821400
E-mail: [email protected]
Received date: January 23, 2016; Accepted date: February 22, 2016; Published date: February 24, 2016
Citation: Ghalsasi VV, Sourjik V (2016) Engineering Escherichia coli to Disrupt Poly-N-Acetylglucosamine Containing Bacterial Biofilms. Curr Synthetic Sys Biol 4:130. doi:10.4172/2332-0737.1000130
Copyright: © 2016 Ghalsasi VV, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Biofilms are surface-associated structures formed by bacteria embedded in a self-produced matrix. Biofilms are exceptionally resistant to environmental stress, antimicrobial agents and host immune defense, and combating biofilms has recently become an important research topic. In this work, we present a biologically engineered system that can be applied against a wide range of biofilms formed by pathogenic and non-pathogenic bacteria. This system relies on Escherichia coli (disrupter) strain that was engineered to synthesize and secrete Dispersin B, an enzyme that can hydrolyze poly-N-acetyl glucosamine (PGA), a polymer found in the matrix of various bacterial biofilms. We show that the degradation of PGA by the disruptor strain results in the dispersion of the target biofilm. We propose that in the future this simple disrupter module can be combined with other biofilm detection and targeting systems aimed towards the destruction of an existing biofilm.