Enhanced Bioactivity of Titanium by Laser-generated Lotus-Topographies:
Molecular Insights in Osteogenic Signaling Pathways of hASCs
Fadeeva E1, Deiwick A1, Chichkov B1,2 and Schlie-Wolter S1,2*
1Laser Zentrum Hannover e.V.; Nanotechnology Department; Hollerithallee 8; D-30419, Hannover, Germany
2Institute of Quantum Optics; Leibniz University Hannover; Welfengarten 1; D-30167, Hannover, Germany
- *Corresponding Author:
- Sabrina Schlie-Wolter
Laser Zentrum Hannover e.V., Hollerithallee 8
30419 Hannover, Germany
E-mail: [email protected]
Received Date: October 18, 2016; Accepted Date: October 24, 2016; Published Date: October 28, 2016
Citation: Fadeeva E, Deiwick A, Chichkov B, Schlie-Wolter S (2016) Enhanced Bioactivity of Titanium by Laser-generated Lotus-Topographies: Molecular Insights in Osteogenic Signaling Pathways of hASCs. J Nanomed Nanotechnol 7: 403. doi: 10.4172/2157-7439.1000403
Copyright: © 2016 Fadeeva E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Laser-generated lotus-topographies were shown to fulfill important requirements of demanded bioactivity: inhibition of biofilms and fibrotic capsule formation and enhanced regeneration. Here, we figure out how the demonstrated markerspecific improved osteogenic differentiation of human adipose-derived stem cells (hASCs) is accomplished. Comparing flat and lotus-structured titanium, the role of the osteogenic signaling pathways via extracellular-signal related kinase (ERK), c-Jun-N-terminal kinase (JNK), and p38 mitogen-activated protein kinases (MAPKs), and interplay with ERK/ p-ERK and bone morphogenic protein (BMP2)/p-Smad signaling are determined. To our knowledge, this interaction has not been analyzed before. Blocking of ERK and JNK suppresses osteogenic markers like alkaline phosphatase (ALP) activity and calcium mineralization independently from the surface. Their functionality requires both transcription factors osterix and Runx2/p-Runx2, and interplay with ERK/p-ERK and BMP2/p-Smad. P38 plays a regulatory role enabling osteogenic differentiation, which depends on the surface design. Its inhibition generally increases ERK/p-ERK cascade resulting in a high extent of Runx2/p-Runx2. Surface dependent BMP2/p-Smad and osterix are activated. The chief cause in enhanced osteogenic differentiation correlates with the improved attachment on the lotus-topography. Therefore, laser-generated lotus-structures present an attractive surface functionalization, finding their application in diverse biomedical applications.