alexa Enhanced Volume of Stem Cell-Based Regenerated Tissue Achieved by Altering Microenvironment and Cell Seeding Strategies | OMICS International | Abstract
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Enhanced Volume of Stem Cell-Based Regenerated Tissue Achieved by Altering Microenvironment and Cell Seeding Strategies

Ricardo A Rosselló1,2,4* and David H Kohn2,3

1Departments of Biochemistry, University of Puerto Rico, Medical Sciences Campus, San Juan, Puerto Rico

2Departments of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA

3Departments of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI, USA

4Department of Science and Technology, Metropolitan University, San Juan, Puerto Rico

Corresponding Author:
Ricardo A Rossello
Department of Biochemistry
University of Puerto Rico
Medical Sciences Campus, San Juan, Puerto Rico
Tel: +1 787-754-0101
E-mail: [email protected]

Received date: October 27, 2013; Accepted date: April 20, 2014; Published date: April 23, 2014

Citation: Rosselló RA, Kohn DH (2014) Enhanced Volume of Stem Cell-Based Regenerated Tissue Achieved by Altering Microenvironment and Cell Seeding Strategies. J Tissue Sci Eng S1:005. doi:10.4172/2157-7552.S1-005

Copyright: © 2014 Rosselló AR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Tissue engineering has emerged as a potential alternative or complementary solution to organ failure or damage. Adult stem cell based approaches can provide a powerful platform for regeneration, given these cells capacity to differentiate into multiple tissues, given the appropriate signals. However, a barrier in the development of tissue in 3D constructs is the transport limitations of nutrients and by products to the core, producing low volumes of regenerated tissue. The microenvironment in the immediate vicinity of the cells, the number of cells that adhere to the substrate and their localization may play an important role in differentiation and tissue regeneration. In this study, we aim to overcome compromised development of hard tissue using 3D cell-based tissue engineering strategies. Specifically, we quantified the effect of a biomimetic template and cell seeding techniques in Gap Junction Intercellular Communication (GJIC), differentiation (Osteocalcin and Alkaline Phosphatase mRNA) In-vitro and bone volume fraction (In-vivo) of adult stem cells (Bone Marrow Stromal Cells) seeded in 3D rigid scaffolds. Significant increases in amount and distribution of bone were achieved when altering both the template and initial seeding conditions. Our findings indicate that creating a biomimetic environment and altering initial seeding conditions that enhance cell adhesion and cell-cell communication in rigid scaffolds are powerful strategies to overcome the incomplete regeneration of cell-based engineered tissue.


Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7