Enhancement of Vibriosis Resistance in Litopenaeus vannamei by Supplementation of Biomastered Silver Nanoparticles by Bacillus subtilisElayaraja Sivaramasamy1,2, Wang Zhiwei1,3, Fuhua Li1 and Jianhai Xiang1*
- *Corresponding Author:
- Jianhai Xiang
Institute of Oceanology, Chinese Academy of Sciences 7
Nanhai Road, Qingdao 266071, China
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E-mail: [email protected]
Received Date: January 14, 2016 Accepted Date: February 05, 2016 Published Date: February 12, 2016
Citation: Sivaramasamy E, Zhiwei W, Li F, Xiang J (2016) Enhancement of Vibriosis Resistance in Litopenaeus vannamei by Supplementation of Biomastered Silver Nanoparticles by Bacillus subtilis. J Nanomed Nanotechnol 7:352. doi: 10.4172/2157-7439.1000352
Copyright: © 2016 Sivaramasamy E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
virulent and most dreadful viral outbreaks in shrimp culture. The potential antibacterial effects of Bacillus subtilis silver nanoparticles (AgNPs) from the gut of Litopenaeus vannamei were studied. The AgNPs has ~ 420 nm in UV-visible spectrum, diameter 5-25 nm with smooth spherical shape (characterized by transmission electron microscopy) and 2θ values corresponding to the presence of silver crystal (X-ray diffraction spectrum). The AgNPs showed promising activity against Vibrio parahaemolyticus (21.25 ± 2.55 mm) and V. harveyi (19.27 ± 1.36 mm) as compared with B. subtilis, silver nitrate (AgNO3) and control. Four different experiments were conducted using different feeding behavior of L. vannamei. In comparison to untreated control group, final weight (14.89 ± 0.03 g), weight gain (9.36 ± 0.01), specific growth rate (SGR) 14.41 ± 0.09%, feed conversion ratios (FCR) 1.47 ± 0.12, higher survival and haemocyte counts were significantly greater in shrimp fed with AgNPs. The gill of entire experimental animal showed morphological alteration in histopathological investigation. The AgNPs were then tested for shrimp challenged with the V. parahaemolyticus. Statistical analysis revealed significant differences in shrimp survival between AgNPs, B. subtilis and control group. In the infective experimental study, cumulative survival of the control group (10 ± 0.321%) whereas the shrimp with AgNPs (90.66 ± 0.523%) and (71 ± 0.577%) with B. subtilis. Subsequently, real-time PCR was observed for immune related genes to determine the mRNA levels of prophenoloxidase (proPO), anti lipopolysaccharide factor (ALF2 and 4), peroxinectin (PE), superoxide dismutase (SOD), 18S, lipopolysaccharide and β -1,3-glucan-binding protein (LGBP ) and serine protein (SP). The expression of all immune related genes (mRNA levels) studied was significantly upregulated in the AgNPs diet shrimp in contrast to the B. subtilis and control. This study discovers that the biomastered AgNPs give a promising potential new tool for inhibiting vibriosis in shrimp culture.