alexa Enrichment and Characterization of Two Subgroups of Committed Osteogenic Cells in the Mouse Endosteal Bone Marrow with Expression Levels of CD24
ISSN: 2572-4916

Journal of Bone Research
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Research Article

Enrichment and Characterization of Two Subgroups of Committed Osteogenic Cells in the Mouse Endosteal Bone Marrow with Expression Levels of CD24

Ching-Fang Chang1,2, Ke-Hsun Hsu1,2, Chia-Ning Shen1,2,3, Chung-Leung Li2,3* and Jean Lu1,2*

1Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan

2Genomics Research Center, Academia Sinica, Taipei, Taiwan

3Institute of Bioscience and Biotechnology, College of Life Sciences, National Taiwan Ocean University, Keelung, Taiwan

*Corresponding Author:
Jean Lu
Genomics Research Center, Academia Sinica, 128 Academia Road, Sec2
IBMS, N401F, Nankang, Taipei 115, Taiwan
Tel: 886-2-2789-9580 ext 601
Fax: 886-2-2789-9587
E-mail: [email protected]
 
Chung-Leung Li
Genomics Research Center, Academia Sinica, Taipei, Taiwan
Tel: 886-2-2789-9580
E-mail: [email protected]

Received May 09, 2014; Accepted September 01, 2014; Published September 03, 2014

Citation: Chang CF, Hsu KH, Shen CN, Li CL, Lu J (2014) Enrichment and Characterization of Two Subgroups of Committed Osteogenic Cells in the Mouse Endosteal Bone Marrow with Expression Levels of CD24. J Bone Marrow Res 2: 144. doi: 10.4172/2329-8820.1000144

Copyright: © 2014 Chang CF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Primary osteogenic cells have been known to reside within the CD45-CD31-Ter119-Sca-1- cell fraction, particularly in the CD51+ subpopulation. However, detailed determination of the frequency of osteogenic cells within this Sca-1- cell population remains yet to be determined. In addition, it is not clear that other cell surface markers can be used to further sub-fractionate this Sca-1-CD51+ osteogenic cell population and to define their developmental stages. In this report, both Sca-1-CD24med and Sca-1- CD24-/lo cells have been shown to be two small subsets of the Sca-1-CD51+ cell fraction. These two cell fractions show subtle difference in the expression level of osteogenic marker genes such as Osx and Opn, and in vitro proliferate rate. All these observations suggest that they may be at different developmental stages of osteogenesis.
The Sca-1-CD24med cell fraction is enriched for the more mature osteolineage cells than the Sca-1-CD24-/lo counterpart. In contrast, most of the Sca-1-CD24hi and Sca-1+CD24-/lo cells do not contain CFU-ALP nor express osteogenic gene markers. The high proliferation ability and osteo-adipogenic differentiation potentials confirm that the Sca-1+CD24-/lo cells are the multipotential mesenchymal stromal cells. The determination of individual stromal cell subpopulations will lead to a better understanding in the hierarchical organization of these osteolineage cells.

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