alexa Enzymatic Extraction of Amino Acids from Fish Waste for Possible Use as a Substrate for Production of Jadomycin | OMICS International | Abstract
ISSN: 2329-6674

Enzyme Engineering
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Enzymatic Extraction of Amino Acids from Fish Waste for Possible Use as a Substrate for Production of Jadomycin

Ramakrishnan V, Ghaly AE*, Brooks MS and Budge SM
Department of Biological Engineering, Faculty of Process Engineering and Applied Science, Sexton Campus, Dalhousie University, Halifax, Nova Scotia, Canada
Corresponding Author : Dr. Abdel Ghaly
Department of Biological Engineering
Faculty of Process Engineering and Applied Science
Dalhousie University, Halifax, Nova Scotia, Canada
Tel: 902-494-6014
E-mail: [email protected]
Received April 05, 2013; Accepted April 09, 2013; Published April 16, 2013
Citation: Ramakrishnan V, Ghaly AE, Brooks MS, Budge SM (2013) Enzymatic Extraction of Amino Acids from Fish Waste for Possible Use as a Substrate for Production of Jadomycin. Enz Eng 2: 112. doi:10.4172/2329-6674.1000112
Copyright: © 2013 Ramakrishnan V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The enzymatic extraction of amino acids from dried fish protein was carried out using the enzymes Alcalase and Neutrase (individually and in combination), and the effect of reaction time (24 and 48 h) on the hydrolysis process was evaluated. The profiling of amino acids was carried out using gas chromatography. During protein hydrolysis, the enzyme Alcalase (being a serine endopeptidase) and the enzyme Neutrase (being a neutral metallo endoprotease) act upon the peptide bonds to release amino acids into the system. The highest amount of amino acids were obtained from the samples hydrolyzed, using a combination of enzymes (Alcalase+Neutrase) for 48 h. Fourteen amino acids were identified. These were: Alanine (7.59%), Glycine (5.82%), Histidine (3.59%), Isoleucine (5.30%), Leucine (9%), Lysine (7.34%), Methionine (2.2%), Phenylalanine (4.2%), Serine (4.3%), Threonine (5.40%), Tyrosine (3.17%), Valine (7.2%), Glutamic acid (9.85%) and Proline (0.98%). Two amino acids (arginine and aspartic acid) could not be quantified, as the enzymes were not able to cleave them. All the amino acids, suitable for the jadomycin production, can be obtained from enzymatic hydrolysis of fish proteins. All the amino acids were above the minimum concentration of 0.45% for the jadomycin production.Tryptophan, which is suitable of producing jadomycin, was not present in the fish protein. The feasibility of jadomycin production from Glutamic acid and Proline should be investigated. Currently, jadomycins are produced individually using only one amino acid in the medium, and as such the amino acids extracted from fish protein have to be separated and purified before use for production of individual jadomyicns. The possibility of multiple production of jadomycins in the same medium, containing the mixture of amino acids, has to be investigated.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version