alexa Enzyme-Modified Screen-Printed Electrodes for Assaying Glucose, Ethanol, Lactate and Starch in Fermentation Media
ISSN: 2167-7972

Fermentation Technology
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Research Article

Enzyme-Modified Screen-Printed Electrodes for Assaying Glucose, Ethanol, Lactate and Starch in Fermentation Media

Kamanin SS1, Arlyapov VA1, Alferov VA1 and Reshetilov AN1,2*

1Federal State Budgetary Institution of Higher Professional Education, Tula State University, 92 Lenin Prosp., Tula, Russia

2Federal State Budgetary Institution of Science, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow, Russia

*Corresponding Author:
Reshetilov AN
Federal State Budgetary Institution of Higher Professional Education
Tula State University
92 Lenin Prosp, Tula, Russia
Tel: +7 926 573 73 62
Fax: +7 495 956 33 70
E-mail: [email protected];

Received date: October 27, 2015; Accepted date: March 24, 2016; Published date: March 31, 2016

Citation: Kamanin SS, Arlyapov VA, Alferov VA, Reshetilov AN (2016) Enzyme-Modified Screen-Printed Electrodes for Assaying Glucose, Ethanol, Lactate and Starch in Fermentation Media. Ferment Technol 5:128. doi: 10.4172/2167-7972.1000128

Copyright: © 2016 Kamanin SS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Screen-printed electrodes modified by glucose oxidase, alcohol oxidase, lactate oxidase and γ-amylase immobilized into cross linked bovine serum albumin gel were produced for application in monitoring fermentation processes. The analytical and metrological characteristics of biosensors based on the developed electrodes were determined. Samples of fermentation mass and alcohol products were assayed. Statistical analysis of the results showed the values of glucose, ethanol, lactate and starch contents, determined using the developed multi-channel system and by reference methods, to differ insignificantly.

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