Epidemiological Study of AGT Gene Polymorphism among Chinese Subjects with Primary Hypertension
|Ning Zhang*, Lili Yang, Huadong Cui and Wenyi Fu|
|Department of Internal Medicine, ShengJing Hospital, China Medical University, Sanhao street36, Heping district, Shenyang, P.R. China|
|*Corresponding Author :||Ning Zhang, Prof.
Dept. of Internal Medicine
Shengjing Hospital of China Medical University
Fax: 0086-24- 23513451
E-mail: [email protected]
|Received March 12, 2012; Accepted May 12, 2012; Published May 15, 2012|
|Citation: Zhang N, Yang L, Cui H, Fu W (2012) Epidemiological Study of AGT Gene Polymorphism among Chinese Subjects with Primary Hypertension. Adv Pharmacoepidem Drug Safety 1:110. doi:10.4172/2167-1052.1000110|
|Copyright: © 2012 Zhang N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Background: Polymorphisms in the promoter region of the angiotensinogen (AGT) gene may affect AGT transcription and thus blood pressure. We determined the frequency of the AGT A-20C polymorphism in Chinese patients with primary hypertension. Using a molecular epidemiology approach, we also determined the relationship between primary hypertension and environmental-AGT A-20C polymorphism interactions. Methods: 912 subjects diagnosed with primary hypertension were analyzed from Shenyang Chinese Populationsand their samples were genotyped using A-20C polymorphism within angiotensinogen (AGT) gene by Ã´ÂÂÂµestriction Ã´ÂÂÂ©ragment Ã´ÂÂÂ¯ength Polymorphism methods (PCR-RFLP)
Results: PCR and restriction fragment length polymorphism techniques were used to determine the distribution frequency of AGT A-20C alleles. Frequencies of 84.7% and 15.3% were observed for the A and C alleles, respectively. The polymorphism was in Hardy-Weinberg equilibrium according to a χ2 test (χ2=0.58, P>0.05). Linear regression of AGT genotype and blood pressure revealed that systolic blood pressure was significantly higher for AC and CC genotypes compared to the wild-type AA genotype in females (P<0.05).
Conclusion: This is the first report on the frequency of AGT A-20C polymorphism in a Chinese population. Compared to the AA genotype, the effects of genotypes AC and CC on blood pressure were mainly manifest as significantly higher systolic blood pressure in females.