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ISSN: 2168-9547

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Research Article

Epitope Mapping of Tobacco Mosaic Virus Capsid Protein: Prediction and Experimental Data from Spot Synthesis

Daniel Ferreira de Lima Neto1, Clarice Weis Arns1, Dagmar Ruth Stach-Machado2, Fernando Rosado Spilki3, Juliana Mattoso4 and Carlos Francisco Sampaio Bonafe4*

1Departamento de Genética, Laboratório de Virologia Animal, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, Rua Monteiro Lobato, 255, Campinas, Brazil

2Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, Rua Monteiro Lobato, 255, Campinas,Brazil

3Laboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, Brazil

4Departamento de Bioquímica, Laboratório de Termodinâmica de Proteínas, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, Rua Monteiro Lobato, 255, Campinas, Brazil

*Corresponding Author:
Dr. Carlos Francisco Sampaio Bonafe
Departamento de Bioquímica, Laboratório de Termodinâmica de Proteínas
Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP)
Cidade Universitária Zeferino Vaz, Rua Monteiro Lobato, 255
Campinas, 13083-862, SP, Brazil
Tel: +55 19 35216229
Fax: +55 19 35216129
E-mail: [email protected]

Received date October 03, 2012; Accepted date October 22, 2012; Published date October 24, 2012

Citation: de Lima Neto DF, Arns CW, Stach-Machado DR, Spilki FR, Mattoso J, et al. (2013) Epitope Mapping of Tobacco Mosaic Virus Capsid Protein: Prediction and Experimental Data from Spot Synthesis. Mol Biol 2:108. doi:10.4172/2168-9547.1000108

Copyright: © 2013 de Lima Neto DF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The immune system is a network of thousands of molecules, cells and regulatory factors that produce many interrelated responses. In this study, we used spot synthesis to map tobacco mosaic virus (TMV) epitopes in a mice animal model (Balb/c) and compared the results with those obtained using immunoinformatic prediction tools. Mice were inoculated with TMV and after immunization the sera were incubated with an array of overlapping pentadecapeptides that corresponded to the full sequence of the TMV capside protein (TMVcp) that had been synthesized on a cellulose membrane for spot synthesis analysis. Six linear epitopes were identified experimentally, as shown by the IgG-elicited immune responses in mice. The data for epitope prediction based on epitope databases agreed with the results obtained by spot synthesis results. Comparison of the findings for spot intensities and those obtained with the prediction software allowed the identification of different responses according to the MHC class I alleles. The results of this work provide a detailed antigenic profile for TMV.

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