alexa Establishment of an Analytical Method for Sialyl Glycoprotein Extraction from the Experimental Hormone-Induced Estrous Cycle of the Mouse Ovary | OMICS International | Abstract
ISSN: 2153-0637

Journal of Glycomics & Lipidomics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Special Issue Article

Establishment of an Analytical Method for Sialyl Glycoprotein Extraction from the Experimental Hormone-Induced Estrous Cycle of the Mouse Ovary

Mari Yokoyama1,3, Yasuhiro Takegawa1 and Tadashi Yamashita1,2*

1Graduate School of Advanced Life Science, Hokkaido University, N21, W11, Sapporo 001-0021, Japan

2World Class University Program, Kyungpook National University School of Medicine, Daegu, South Korea

3Discovery Research Laboratories, Shionogi & Co. Ltd., Toyonaka-shi, Osaka 561–0825, Japan

*Corresponding Author:
Dr. Tadashi Yamashita
Graduate School of Advanced Life Science
Hokkaido University, N21, W11
Sapporo 001-0021, Japan
Tel: +81- (11)-706-9084
Fax: +81-(11)-706-9002
E-mail: [email protected]

Received date: February 05, 2012; Accepted date: February 13, 2012; Published date: February 15, 2012

Citation: Yokoyama M, Takegawa Y, Yamashita T (2012) Establishment of an Analytical Method for Sialyl Glycoprotein Extraction from the Experimental Hormone-Induced Estrous Cycle of the Mouse Ovary. J Glycom Lipidom S4:001. doi: 10.4172/2153-0637.S4-001

Copyright: © 2012 Yokoyama M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Ovaries are often found in pairs as part of the vertebrate female reproductive system that maintains immature egg cells until they become mature with ovulation. The ovary is also an internal secretion organ for both estrogen and progesterone. Estrogen is responsible for the appearance of secondary sex characteristics of females at puberty and for the maturation and maintenance of the reproductive organs. Progesterone functions mainly to regulate the condition of the endometrium, preparing it to accept a fertilized egg. On the other hand, glycans are a main component of the animal cell surface and show structural changes during early development and cell differentiation. In the present study, to clarify the relation between the changes in glycan structures and ovarian functions such as ovulation, we used a system in which ovulation was induced by sex hormone treatment. As a result, it was found that glycoproteins including terminal sialic acids, especially N-glycolyl neuraminic acid-type glycans, increased during development of the ovary. Furthermore, to find specific glycoproteins with the N-glycolyl neuraminic acid-type glycan that were induced by hormone treatment, we established a method for the purification of glycoproteins, using sialic acid followed by detection using mass spectrometry. Applying the modified protocol, transferrin could be detected as the protein that changed the amount of glycans in the developmental cycle of the ovary. Thus, this protocol will be a useful tool for the detection of glycoproteins with sialic acid residues. It may also be a valuable method for use with other organs.

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version