alexa Establishment of Efficient Regeneration System from Leaf Discs in Long Pepper an Important Medicinal Plant (Piper longum L.)
ISSN: 2167-0412

Medicinal & Aromatic Plants
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Research Article

Establishment of Efficient Regeneration System from Leaf Discs in Long Pepper an Important Medicinal Plant (Piper longum L.)

Sathelly K1,2, Podha S2, Pandey S1, Mangamuri U3 and Kaul T1*

1Nutritional Improvement of Crops Group - Plant Biology, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110 067, India

2Department of Biotechnology, Acharya Nagarjuna University, Guntur-522 510, Andhra Pradesh, India

3Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur-522 510, Andhra Pradesh, India

Corresponding Author:
Kaul T
Nutritional Improvement of Crops Group - Plant Biology
International Centre for Genetic Engineering and Biotechnology
Aruna Asaf Ali Marg, New Delhi-110 067, India
Tel: +911126742962
E-mail: [email protected]

Received: March 24, 2016; Accepted: October 09, 2015; Published: April 26, 2016

Citation: Sathelly K, Podha S, Pandey S, Mangamuri U, Kaul T (2016) Establishment of Efficient Regeneration System from Leaf Discs in Long Pepper an Important Medicinal Plant (Piper longum L.). Med Aromat Plants 5:248. doi:10.4172/2167-0412.1000248

Copyright: © 2016 Sathelly K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Cultivation of Piper longum L. till recently was not very common; still it is extensively collected from the wild, threatening the very existence of the plant. Hence, it is alarming that there is an urgent need not only cultivate this plant but also develop conservation strategies. Conventional propagation is overwhelmed with problems of poor seed viability low percentage of germination, scanty and delayed rooting of vegetative cuttings indicating there is a need for alternative propagation methods. In vitro technique is an alternative approach to solve the problem. Therefore, the current research was aimed at developing a promising in vitro mass propagation protocol for Piper longum L. using leaf as explant. Murashige and Skoog medium was used throughout the experiment. For callus induction, MS medium supplemented with alone or in combination of IAA (1 to 2 mg/l) and BAP (1 to 2 mg/l) were used. Shoot induction was undertaken on MS medium supplemented with different concentrations and combinations of Kinetin and BAP (1 to 3 mg/l). Emerged shoots were transferred onto elongation medium supplemented with 2 mg/l Zeatin and 1 mg/l GA3. In vitro rooting was achieved on ½ MS medium+NAA 1 mg/l. Accordingly, the highest calli were induced on MS+1 mg/L IAA+1 mg/L BAP. Among the various treatments, the maximum percentage (91.50 ± 3.54) in vitro shooting was observed on MS+2 mg/L BAP+1 mg/L Kinetin. In vitro rooted shoots were successfully acclimatized in the greenhouse conditions. Therefore, it is possible to deduce that the current protocol is promising for in vitro mass propagation of Piper longum L. to solve the reproduction and cultivation problem of the plant.


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