Estimation of Effect of Lead, Alcohol and Vitamin E on Aspartate Amino Transferase and Alanine Amino Transferase of Liver Tissue in Rats
Eight groups of rats, each group consists of six animals. Group I acts as control receiving water. Group II were treated with lead acetate at 160mg/ liter concentration dissolved in water. Group III animals were treated with 10% alcohol. Group IV animals were treated with 160 mg/ liter concentration of lead acetate and 10% alcohol. Group V animals served as control treated with 500mg Vitamin E/kg diet. Group VI animals were treated with lead acetate at 160 mg/ liter concentration dissolved in water and Vitamin E/kg diet. Group VII animals were treated with 10% alcohol and Vitamin E/kg diet. Group VIII animals were treated with 160 mg/ liter concentration of lead acetate , 10% alcohol and 500mg Vitamin E /kg diet. Effect of alcohol, lead and vitamin E for eight weeks on AST (Aspartate amino transferase) and ALT (Alanine amino transferase) of liver tissue was evaluated in in vitro condition. When treated with lead, the AST was recorded 92.50U/L, 100.0 U/L in alcohol, 119.67 U/L in lead(160mg/ liter) with alcohol(10%). The AST was 54.50U/L in vitamin E (500mg/kg diet) treated tissue. 76.75 U/L in lead and vitamin E treated tissue, 85.75U/L in alcohol, vitamin E treated tissue and 102.25U/L in lead, alcohol and vitamin E treated liver tissue. ALT recorded 50.50 U/L in lead treated tissue, 53.25 U/L in alcohol treated tissue, 67.75 U/L in lead with alcohol treatment, 32.24 U/L in vitamin treated liver tissue, 37.33 U/L in lead with vitamin E treated tissue, 51.75 U/L in alcohol and vitamin treated tissue and 57.0 U/L in lead, alcohol and vitamin E treated liver tissue.