alexa Estimation of Metformin in Bulk Drug and in Formulation
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

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Research Article

Estimation of Metformin in Bulk Drug and in Formulation by HPTLC

Shweta Havele and Sunil Dhaneshwar*

Research and Development Centre in Pharmaceutical Sciences and Applied Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune – 411038, India

*Corresponding Author:
Sunil Dhaneshwar
Research and Development Centre in Pharmaceutical
Sciences and Applied Chemistry
Poona College of Pharmacy
Bharati Vidyapeeth University
Erandwane, Pune – 411038, India
E-mail: [email protected]

Received Date: October 02, 2010; Accepted Date: October 25, 2010; Published Date: October 27, 2010

Citation: Havele S, Dhaneshwar S (2010) Estimation of Metformin in Bulk Drug and in Formulation by HPTLC. J Nanomedic Nanotechnolo 1:102. doi:10.4172/2157-7439.1000102

Copyright: © 2010 Havele S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



A simple and sensitive, HPTLC method has been developed for the quantitative estimation of metformin in its single component tablet formulation. Metformin was chromatographed on silica Gel 60 F 254 TLC plate using ammonium sulfate (0.5%): 2-propanol: methanol in the ratio of 8.0:1.6:1.6 (v/v/v) as mobile phase. Metformin showed R f value of 0.50±0.03 was scanned at 238 nm using Camag TLC Scanner 3. The linear regression data for the calibration plot showed a good relationship with r =0.999. The method was validated for precision and recovery. The limits of detection and quanti fi cation were 95 and 200 ng/spot respectively. The developed method was successfully used for the assay of metformin tablet formulations. The method is simple, sensitive and precise; it can be used for the routine quality control testing of marketed formulations.

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