alexa Estimation of Metformin in Bulk Drug and in Formulation by HPTLC | OMICS International | Abstract

Research Article

Estimation of Metformin in Bulk Drug and in Formulation by HPTLC

Shweta Havele and Sunil Dhaneshwar*

Research and Development Centre in Pharmaceutical Sciences and Applied Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune – 411038, India

*Corresponding Author:
Sunil Dhaneshwar
Research and Development Centre in Pharmaceutical
Sciences and Applied Chemistry
Poona College of Pharmacy
Bharati Vidyapeeth University
Erandwane, Pune – 411038, India
E-mail: [email protected]

Received Date: October 02, 2010; Accepted Date: October 25, 2010; Published Date: October 27, 2010

Citation: Havele S, Dhaneshwar S (2010) Estimation of Metformin in Bulk Drug and in Formulation by HPTLC. J Nanomedic Nanotechnolo 1:102. doi:10.4172/2157-7439.1000102

Copyright: © 2010 Havele S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A simple and sensitive, HPTLC method has been developed for the quantitative estimation of metformin in its single component tablet formulation. Metformin was chromatographed on silica Gel 60 F 254 TLC plate using ammonium sulfate (0.5%): 2-propanol: methanol in the ratio of 8.0:1.6:1.6 (v/v/v) as mobile phase. Metformin showed R f value of 0.50±0.03 was scanned at 238 nm using Camag TLC Scanner 3. The linear regression data for the calibration plot showed a good relationship with r =0.999. The method was validated for precision and recovery. The limits of detection and quanti fi cation were 95 and 200 ng/spot respectively. The developed method was successfully used for the assay of metformin tablet formulations. The method is simple, sensitive and precise; it can be used for the routine quality control testing of marketed formulations.

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