alexa Estimation of Nevirapine from Human Plasma by ESI-LC-MS/MS: a Pharmacokinetic Application
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

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Research Article

Estimation of Nevirapine from Human Plasma by ESI-LC-MS/MS: a Pharmacokinetic Application

Chinmoy Ghosh1,2, Shashank Gaur1, Ajay Singh1, Chandrakanta P. Shinde2 and Bhaswat S. Chakraborty1*

1Bio-analytical Department, Cadila Pharmaceuticals Limited, 1389-Trasad road, Dholka, Gujarat, India

2Jiwaji University, School of studies in Chemistry, Gwalior, M.P., India

*Corresponding Author:
Dr. Bhaswat S. Chakraborty
Sr. Vice President
Bioanalytical Department
Contract Research Organization
Cadila Pharmaceuticals Limited
1389, Trasad road, Dholka-387 810
Dist: Ahmedabad, Gujarat, India
Tel: +91-2714-221481/83/84 Ext. 107
Fax: +91-2714-221848
Email: [email protected]

Received Date: January 05, 2011; Accepted Date: January 24, 2011; Published Date: February 08, 2011

Citation: Ghosh C, Gaur S, Singh A, Shinde CP, Chakraborty BS (2011) Estimation of Nevirapine from Human Plasma by ESI-LC-MS/MS: a Pharmacokinetic Application. J Bioequiv Availab 3: 020-025. doi: 10.4172/jbb.1000052

Copyright: © 2011 Ghosh C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A selective, sensitive, and fast High Performance Liquid Chromatography with mass spectrometric (MS) detection method was developed and validated in human plasma. Nevirapine and Enalapril (internal standard, IS) were extracted from human plasma via solid phase extraction (SPE) technique. After the elution through SPE cartridge, samples were directly analyzed using LC-ESI-MS/MS system. An isocratic mode is used to separate interference peaks using a hypurity advanced C-18, 50 X 4.6 mm ID, 5μ, column. The mobile phase composition was 0.1% formic acid in Milli-Q water (v/v) to acetonitrile at a ratio of 15:85, v/v.The m/z of nevirapine and IS were 267.00 /226.20 and 377.10 /234.10, respectively. Linearity ranges were 10.00 to 5000.50 ng/mL. Calibration functions, lower limit of quantitation (LLOQ), stability, intra- and inter-day reproducibility, accuracy, and recovery are estimated. This method was free from matrix effects and any abnormal ionization. This method was successfully applied to a pharmacokinetic study of nevirapine.

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