Estimation of Rutin and Ascorbic Acid in Some Libyan Herbal Plants by RP-HPLC
- *Corresponding Author:
- Habib HII
Faculty of Science
Omar El-Mukhtar University
E-mail: [email protected]
Received date: March 25, 2016; Accepted date: June 10, 2016; Published date: June 13, 2016
Citation: Habib HII, Omar SK, Mohamed HS (2016) Estimation of Rutin and Ascorbic Acid in Some Libyan Herbal Plants by RP-HPLC. Med Aromat Plants 5: 255. doi:10.4172/2167-0412.1000255
Copyright: © 2016 Habib HII, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: A combination of rutin and ascorbic acid were exploited in several pharmaceutical activities and so simple and high sensitive liquid chromatographic method for the simultaneous determination of the cited ingredients in methanolic extracts of different Libyan herbal plants was proposed.
Methods: The chromatographic analysis was performed using 250 × 4.6 mm Brownlee BIO C18 column, 5 μm particle size, with UV detection at 254 nm and isocratic elution of methanol–phosphate buffer pH 3.2, which is initially in 45:55 (v/v) for 0.5 min (equilibrium state), then varied to 60:40 (v/v) isocratic for 8 min and then back to 45:55 for 2 min, at a flow rate 1 ml/min and at ambient temperature.
Results: The percentages of rutin and ascorbic acid found were 0.7, 0.1 in Salvia fruticosa, 1.72, 2.31 in Thymus vulgaris, 0.44, 0.35 in Rosemary officinalis, 2.87, 3.56 in Matricaria chamomilla L., 2.10, 0.62 in Artemisia absinthium respectively.
Conclusion: The proposed method was suitable for the identification and quantification of the binary combination of rutin and ascorbic acid in Libyan herbal plants.