Estimation of the True Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Autoantibody Concentration in the Sera of Normal Subjects
Received Date: Oct 30, 2017 / Accepted Date: Nov 14, 2017 / Published Date: Nov 21, 2017
According to our previous studies, Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) autoantibodies (GMAbs) are normally present in healthy people. To date, antigen-antibody capture assay has measured the concentrations. However, nonspecific IgG other than GMAbs and immune complex formation with intrinsic GM-CSF prevents us from measuring the accurate concentrations. To solve these problems, we developed a liquid phase high-sensitivity assay using biotinylated GM-CSF (bGM-CSF) and streptavidin coated plate, which could measure the concentration of GMAbs over 0.02 ng/ml. Using this method, the true GMAbs concentration in 30 normal subjects was estimated to be 52.6 ng/ml for the median value, ranging 10.6 to 799.5 ng/ml. Thus, we propose a novel method that enables us to estimate the true concentration of GMAbs in the sera of healthy subjects.
Keywords: Pulmonary alveolar proteinosis; Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF); Autoantibody; Liquid phase reaction
Citation: Shiiya K, Iizuka M, Ito Y, Utako S, Atsushi H, et al. (2017) Estimation of the True Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Autoantibody Concentration in the Sera of Normal Subjects. J Bioanal Biomed 9: 272-276. Doi: 10.4172/1948-593X.1000192
Copyright: © 2017 Shiiya K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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