alexa Estimation of the True Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Autoantibody Concentration in the Sera of Normal Subjects | OMICS International| Abstract
ISSN: 1948-593X

Journal of Bioanalysis & Biomedicine
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  • Research Article   
  • J Bioanal Biomed 2017 9: 272,
  • DOI: 10.4172/1948-593X.1000192

Estimation of the True Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Autoantibody Concentration in the Sera of Normal Subjects

Keiko Shiiya1, Mariko Iizuka1, Yuko Ito1, Seino Utako1, Hashimoto Atsushi1, Takahiro Tanaka2, Keiichi Akasaka3, Nobutaka Kitamura2, Yoshikazu Inoue4, Ryushi Tazawa1 and Koh Nakata1,2*
1Bioscience Medical Research Center, Niigata University Medical & Dental Hospital Niigata, , Japan
2Clinical and Translational Research Center, Niigata University Medical & Dental Hospital Niigata, , Japan
3Uonuma General Education Center, Niigata University Medical & Dental Hospital Niigata, , Japan
4NHO-Kinkichuo Chest Medical Center, , Osaka, Japan
*Corresponding Author : Koh Nakata, Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Asahimachi-dori, Niigata, Japan, Tel: +81-25-227-0847, Fax: +81-25-227-0377, Email: [email protected]

Received Date: Oct 30, 2017 / Accepted Date: Nov 14, 2017 / Published Date: Nov 21, 2017

Abstract

According to our previous studies, Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) autoantibodies (GMAbs) are normally present in healthy people. To date, antigen-antibody capture assay has measured the concentrations. However, nonspecific IgG other than GMAbs and immune complex formation with intrinsic GM-CSF prevents us from measuring the accurate concentrations. To solve these problems, we developed a liquid phase high-sensitivity assay using biotinylated GM-CSF (bGM-CSF) and streptavidin coated plate, which could measure the concentration of GMAbs over 0.02 ng/ml. Using this method, the true GMAbs concentration in 30 normal subjects was estimated to be 52.6 ng/ml for the median value, ranging 10.6 to 799.5 ng/ml. Thus, we propose a novel method that enables us to estimate the true concentration of GMAbs in the sera of healthy subjects.

Keywords: Pulmonary alveolar proteinosis; Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF); Autoantibody; Liquid phase reaction

Citation: Shiiya K, Iizuka M, Ito Y, Utako S, Atsushi H, et al. (2017) Estimation of the True Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Autoantibody Concentration in the Sera of Normal Subjects. J Bioanal Biomed 9: 272-276. Doi: 10.4172/1948-593X.1000192

Copyright: © 2017 Shiiya K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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