alexa Ethidium Bromide and SYBR Green I Interact with Lipid Micelles
ISSN: 2150-3494

Chemical Sciences Journal
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Research Article

Ethidium Bromide and SYBR Green I Interact with Lipid Micelles

Garro AG1, Velez PS1, Miotti N2, Alasino RV13* and Beltramo DM1,3,4*

1Centro de Excelencia en Productos y Procesos de Córdoba (CEPROCOR), Santa María de Punilla, Córdoba, Argentina

2Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina

3Consejo Nacional de Investigaciones, Científicas y Técnicas (CONICET), Argentina

4Departamento de Biotecnología de la, Facultad de Ciencias Químicas, Universidad Católica de Córdoba, Argentina

*Corresponding Author:
Roxana V Alasino
Centro de Excelencia en Productos y Procesos de Córdoba (CEPROCOR)
Santa María de Punilla
Córdoba, Argentina
Tel: 543541489651/53
E-mail: [email protected]

Dante M Beltramo
Centro de Excelencia en Productos y Procesos de Córdoba (CEPROCOR)
Santa María de Punilla, Córdoba
Tel: 543541489651/53
E-mail: [email protected]

Received date: April 28, 2017; Accepted date: May 05, 2017; Published date: May 06, 2017

Citation: Garro AG, Velez PS, Miotti N, Alasino RV, Beltramo DM (2017) Ethidium Bromide and SYBR Green I Interact with Lipid Micelles. Chem Sci J 8: 152. doi: 10.4172/2150-3494.1000152

Copyright: © 2017 Garro AG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Ethidium Bromide and SYBR Green I are usual examples of ligands that specifically binds to DNA by intercalation. Here we describe that micelles of gangliosides, a lipidic family of sialoglycosphingolipids which are the most important lipids in central nervous system, are able to interact with both, EtBr and SYBR Green I and fluoresce after UV light excitation. The interaction is not affected by the presence of high saline concentration (1M NaCl), a condition which dissociate potential electrostatic interaction between amino and carboxylic acid groups present in EtBr and GM1 respectively; instead, increase the fluorescense of EtBr/GM1 micelles. This result suggests that the EtBr is located inside the hydrophobic core of the micelle. Moreover, the SYBR Green I associated ganglioside micelles does not emit green light as with DNA, but there was a change in the light spectrum towards higher wave lengths, now emitting orange light. It was also found that the interaction with these dyes is not affected when the micelles were preloaded with other molecules (paclitaxel albumin). Moreover, further studies showed that this interaction is not observed with other ionic and nonionic classic detergent micelles, and even the addition of these molecules to GM1 micelles interferes with the described EtBr / GM1 interaction.


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