alexa Ethidium Bromide and SYBR Green I Interact with Lipid Micelles
ISSN: 2150-3494

Chemical Sciences Journal
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Ethidium Bromide and SYBR Green I Interact with Lipid Micelles

Garro AG1, Velez PS1, Miotti N2, Alasino RV13* and Beltramo DM1,3,4*

1Centro de Excelencia en Productos y Procesos de Córdoba (CEPROCOR), Santa María de Punilla, Córdoba, Argentina

2Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina

3Consejo Nacional de Investigaciones, Científicas y Técnicas (CONICET), Argentina

4Departamento de Biotecnología de la, Facultad de Ciencias Químicas, Universidad Católica de Córdoba, Argentina

*Corresponding Author:
Roxana V Alasino
Centro de Excelencia en Productos y Procesos de Córdoba (CEPROCOR)
Santa María de Punilla
Córdoba, Argentina
Tel: 543541489651/53
E-mail: [email protected]

Dante M Beltramo
Centro de Excelencia en Productos y Procesos de Córdoba (CEPROCOR)
Santa María de Punilla, Córdoba
Argentina
Tel: 543541489651/53
E-mail: [email protected]

Received date: April 28, 2017; Accepted date: May 05, 2017; Published date: May 06, 2017

Citation: Garro AG, Velez PS, Miotti N, Alasino RV, Beltramo DM (2017) Ethidium Bromide and SYBR Green I Interact with Lipid Micelles. Chem Sci J 8: 152. doi: 10.4172/2150-3494.1000152

Copyright: © 2017 Garro AG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Ethidium Bromide and SYBR Green I are usual examples of ligands that specifically binds to DNA by intercalation. Here we describe that micelles of gangliosides, a lipidic family of sialoglycosphingolipids which are the most important lipids in central nervous system, are able to interact with both, EtBr and SYBR Green I and fluoresce after UV light excitation. The interaction is not affected by the presence of high saline concentration (1M NaCl), a condition which dissociate potential electrostatic interaction between amino and carboxylic acid groups present in EtBr and GM1 respectively; instead, increase the fluorescense of EtBr/GM1 micelles. This result suggests that the EtBr is located inside the hydrophobic core of the micelle. Moreover, the SYBR Green I associated ganglioside micelles does not emit green light as with DNA, but there was a change in the light spectrum towards higher wave lengths, now emitting orange light. It was also found that the interaction with these dyes is not affected when the micelles were preloaded with other molecules (paclitaxel albumin). Moreover, further studies showed that this interaction is not observed with other ionic and nonionic classic detergent micelles, and even the addition of these molecules to GM1 micelles interferes with the described EtBr / GM1 interaction.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords