alexa <p>Eukaryotic Plasmids with <em>Toxoplasma gondii</em> Dense Granule Antigen (GRA 5) and Microneme 3 (MIC3) Genes as a Cocktail DNA Vaccine and Evaluation of Immune Responses in BALB/C Mice</p> | Abstract
ISSN: 2472-128X

Journal of Clinical & Medical Genomics
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Research Article

Eukaryotic Plasmids with Toxoplasma gondii Dense Granule Antigen (GRA 5) and Microneme 3 (MIC3) Genes as a Cocktail DNA Vaccine and Evaluation of Immune Responses in BALB/C Mice

Ghaffarifar F1*, Naserifar R2 and Jafari Madrak M3
1Parasitology and Entomology Department, Faculty of Medical Sciences, TarbiatModares University, Tehran, I. R. Iran
2Department of Medical Parasitology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, I. R. Iran
3Department of Medical Parasitology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, I. R. Iran
Corresponding Author : Fatemeh Ghaffarifar
Parasitology and Entomology Department
Faculty of Medical Sciences
TarbiatModares University, Tehran
I.R. Iran, P.O. Box 14115-331
Tel: +982182884553
Fax: +982182884555
E-mail: [email protected]
Received March 30, 2015; Accepted April 11, 2015; Published April 18, 2015
Citation: Ghaffarifar F, Naserifar R, Jafari Madrak M (2014) Eukaryotic Plasmids with Toxoplasma gondii Dense Granule Antigen (GRA 5) and Microneme 3 (MIC3) Genes as a Cocktail DNA Vaccine and Evaluation of Immune Responses in BALB/C Mice. J Clin Med Genomics 3:121. doi: 10.4172/2472-128X.1000121
Copyright: © 2015 Ghaffarifar F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Toxoplasma gondii is an obligate intracellular protozoan that causes toxoplasmosis in human and animal. This parasite is worldwide spread and about one third of people are seropositive. Toxoplasmosis in immuno compromised patients causes serious symptoms. Toxoplasma gondii has a lot of various immunogenic antigens. Excreted/secreted antigens could stimulate of the cell mediated immune response and hence it appears to be a good candidate for vaccine in toxoplasmosis. In this study Microneme3 (MIC3) and GRA5 of Toxoplasma gondii are used as DNA vaccine.

The results indicated that survival rate of mice that immunized by recombinant plasmid have significant differences with control groups. IgG and IgG2a assay approved significant different between case and control groups (P<0.05). Cytokine assay indicated high level of IFN-γ and low level of IL4 for immunized groups.These results indicated DNA vaccine encoded MIC3 and GRA5 genes of Toxoplasma gondii capable to induced partially protection against toxoplasmosis.

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