alexa Evaluating Sensitivity of ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit for Scoring Molecular Response
ISSN: 2155-9864

Journal of Blood Disorders & Transfusion
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Evaluating Sensitivity of ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit for Scoring Molecular Response

Akiko Mita1, Koichi Miyamura2, Masayuki Hino3, Kyoko Watakabe2, Keita Takahashi4, Michiko Yoshimoto4 and Naoto Takahashi1*

1Department of Hematology, Nephrology, and Rheumatology, Akita University, Japan

2Depertment of Hematology, Japanese Red Cross Nagoya First Hospital, Japan

3Department of Hematology, Graduate School of Medicine, Osaka City University, Japan

4Sysmex Corporation, Japan

*Corresponding Author:
Naoto Takahashi
Department of Hematology
Nephrology, and Rheumatology
Akita University, 1-1-1 Hondo
Akita City, Akita-010-8543, Japan
Tel: +81-18-884-6115
Fax: +81-18-836-2613
E-mail: naotot@doc.med.akita-u.ac.jp

Received: September 01, 2015 Accepted date: October 05, 2015 Published date: October 10, 2015

Citation: Mita A, Miyamura K, Hino M, Watakabe K, Takahashi K, et al. (2015) Evaluating Sensitivity of ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit for Scoring Molecular Response. J Blood Disord Transfus 6:314. doi:10.4172/2155-9864.1000314

Copyright: © 2015 Mita A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Objective: Tyrosine kinase inhibitors (TKIs) are the standard of care for patients with chronic myeloid leukemia (CML). Recent studies demonstrated that some patients with CML could sustain remission under discontinuation of TKIs. The clinical decision to discontinue such therapy depends on the patient’s molecular response (MR), derived from the international scale of BCR-ABL1 transcripts per internal control gene transcripts (BCR-ABL1IS), with MR4.5 (i.e., BCR-ABL1IS ≤0.0032%) commonly used as a threshold for discontinuation of TKI. However, it remains controversial whether all methods for determining BCR-ABL1IS have sufficient sensitivity for measuring MR4.5.

Methods: Probit analysis with reference to CLSI EP-17-A2 was used for the kit-specific limit of detection (LoD) of BCR-ABL1 transcripts measured by the ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit. In total, 50 samples of peripheral blood (PB) were collected from CML patients across three sites, with each 21-ml sample split into 7- and 14-ml volumes.

Results: The kit-specific LoD was determined as 3 copies/assay. The sample measurements of 94% (7-ml PB group) and 96% (14-ml PB group) showed over 94 000 copies/assay of ABL1 transcripts, which is the level required to score MR4.5 considering kit-specific LoD of 3 copies/assay.

Conclusion: This result demonstrated that the ipsogen BCR-ABL1 Mbcr IS-MMR DX Kit possesses sufficient sensitivity for stable scoring at MR4.5, with strict conditions set for avoiding false-negative results.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

agriaquaculture@omicsonline.com

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

biochemjournals@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

chemistryjournals@omicsonline.com

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

clinicaljournals@omicsonline.com

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

engineeringjournals@omicsonline.com

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

nutritionjournals@omicsonline.com

1-702-714-7001Extn: 9042

General Science

Andrea Jason

generalscience@omicsonline.com

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

geneticsmolbio@omicsonline.com

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immunomicrobiol@omicsonline.com

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

nursinghealthcare@omicsonline.com

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

medicaljournals@omicsonline.com

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuropsychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

pharmajournals@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords