alexa Evaluating the Diversity of Culturable Thermotolerant Bacteria from Four Hot Springs of India | OMICS International| Abstract

ISSN: 2376-0214

Journal of Biodiversity, Bioprospecting and Development

  • Research Article   
  • J Biodivers Biopros Dev 2014, Vol 1(3): 127
  • DOI: 10.4172/2376-0214.1000127

Evaluating the Diversity of Culturable Thermotolerant Bacteria from Four Hot Springs of India

Murugan Kumar, Ajar Nath Yadav, Rameshwar Tiwari, Radha Prasanna and Anil Kumar Saxena*
Division of Microbiology, Indian Agricultural Research Institute (IARI), New Delhi 110012, India
*Corresponding Author : Anil Kumar Saxena, Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India, Tel: 91 11 25847649, Fax: 91 11 25846420, Email: [email protected]

Received Date: Jul 04, 2014 / Accepted Date: Sep 27, 2014 / Published Date: Sep 29, 2014

Abstract

The culturable bacterial diversity of four hot springs of India was analysed, employing different media and screened for temperature tolerance (40°C - 70°C). Sixty morphotypes from Bakreshwar, 48 from Balrampur, 46 from Chumathang and 52 from Vashist were obtained. A set of 112 isolates, tolerant to 45°C and above, were analysed employing Amplified ribosomal DNA restriction analysis (ARDRA). Sequencing of 16S rRNA gene of the representative isolates revealed that 86%, 93%, 44% and 44% of the isolates respectively from Bakreshwar, Balrampur, Chumathang and Vashist, belonged to Firmicutes. Members of Actinobacteria were present in all the four hot springs, while Proteobacteria were present only in Chumathang and Vashist and Bacteroidetes found only in Bakreshwar. This is the first report of Aurantimonas and Brevundimonas in hot springs. Biolog analyses of three isolates, growing at or above 60°C revealed unique abilities, in terms of utilization of substrates and resistance patterns.

Keywords: ARDRA; Biolog; Culturable bacteria; 16S rRNA; Hot springs; Thermotolerance

Citation: Kumar M, Nath Yadav A, Tiwari R, Prasanna R, Saxena AK (2014) Evaluating the Diversity of Culturable Thermotolerant Bacteria drom Four Hot Springs of India. J Biodivers Biopros Dev 1: 127. Doi: 10.4172/2376-0214.1000127

Copyright: ©2014 Kumar M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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