Evaluating the Nutritional Condition of an Indian Climbing Perch, Anabas Testudineus Fingerlings by the RNA/DNA, Ca/P Ratio and Protein Bio-Synthesis in Liver and MusclePatra BC1*, Patra S2 and Bhattacharya M2
- *Corresponding Author:
- Patra BC
Centre for Aquaculture Research
Extension & Livelihood, Department of Aquaculture Management & Technology
Vidyasagar University, Midnapore-721102, West Bengal, India
Tel: +91 9434185066
E-mail: [email protected]
Received date: February 28, 2017; Accepted date: March 15, 2017; Published date: March 23, 2017
Citation: Patra BC, Patra S, Bhattacharya M (2017) Evaluating the Nutritional Condition of an Indian Climbing Perch, Anabas Testudineus Fingerlings by the RNA/DNA, Ca/P Ratio and Protein Bio-Synthesis in Liver and Muscle. Fish Aqua J 8:193. doi: 10.4172/2150-3508.1000193
Copyright: © 2017 Patra BC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Early fingerlings of Anabas testudineus were nurtured in the laboratory for six weeks under altered nutritional status (different dietary protein level and source) and feeding regimes (ad libitum, ‘limited’, and ‘starvation’ intervals). Their RNA/DNA, Ca/P ratios and protein biosynthesis were determined with highly sensitive fluorescence technique, spectrophotometer and liquid scintillation system respectively. Well-fed early fingerlings (4-6 weeks age group) having higher RNA/DNA (2.11), Ca/P (1.82) ratio and protein biosynthesis rates both in muscle and liver have significantly differs from fingerlings with ‘limited’ food supply and 'starvation' intervals. Rate of protein biosynthesis >244.33 & 603.24 in muscle & liver respectively (count per minute mg protein-1) when compared with well-fed fingerlings and reared under 'limited' food supply and 'starvation' intervals. At ‘starvation’ interval no significant differences were recorded due to different diet treatment. However, fingerlings showed higher mortalities, decreased growth, RNA/DNA, Ca/P ratio and protein biosynthesis rate that may be caused by malnutrition. Fingerlings (early) sampled after 42 days in nearby areas during late monsoon (September) showed RNA/DNA, Ca/P ratio and protein biosynthesis rate were surprisingly low almost as low as ‘starved’ fingerlings, when judged on the basis of mean body weight as compared to well fed fish. It was also observed under microscope that some of these fingerlings were seriously affected by an intestinal disease and this may be due to the ingestion of unsuitable copepods. The low RNA/DNA, Ca/P ratio and protein biosynthesis rate in field samples and ‘starved’ fish are, therefore, interpreted as a sub-lethal result of this disease and malnutrition respectively.