Evaluation of Anti-inflammatory and Anti-proliferative Activity of Abutilon indicum L. Plant Ethanolic Leaf Extract on Lung Cancer Cell Line A549 for System Network Studies
- *Corresponding Author:
- Kaladhar DSVGK
Assistant Professor, Department of Biochemistry/Bioinformatics
GIS, GITAM University, Visakhapatnam-530045, Andhra Pradesh, India
E-mail: [email protected]
Received Date: March 27, 2014; Accepted Date: May 16, 2014; Published Date: May 20, 2014
Citation: Kaladhar DSVGK, Saranya KS, Vadlapudi V, Yarla NS (2014) Evaluation of Anti-inflammatory and Anti-proliferative Activity of Abutilon indicum L. Plant Ethanolic Leaf Extract on Lung Cancer Cell Line A549 for System Network Studies. J Cancer Sci Ther 6: 195-201. doi: 10.4172/1948-5956.1000271
Copyright: © 2014 Kaladhar DSVGK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Pharmaceutically important components in medicinal plant kingdom are useful to control and cure many diseases like lung cancer. In the present study, an evaluation was performed to know the anti-inflammatory and anti-proliferative activity of ethanolic leaf extract of Abutilon indicum for potential chemopreventive agent against lung cancer. Experimentation is also conducted on lung cancer cell line A549 along with interaction studies of Apaf- 1 gene. The ethanolic leaf extract of A. indicum is showing good anti-inflammatory activity (IC50: 8.89 μg/mL) based on 5-Lipoxygenase (5-LOX) inhibition assay. The standard compound, Curcumin has shown IC50 as 8.14 μg/mL based on study. The ethanolic leaf extract of A.indicum has also shown good response on human Caucasian lung carcinoma of A549 cell line (IC50: 85.2 μg/mL) shows anti-proliferative activity. Further analysis has also shown the interaction of proteins that are involved in inflammatory and cancer response. Apoptosis-activating factor, Apaf- 1 gene increases the sensitivity of A549 cell line through interaction with proteins like CASP9, CASP3, CYCS, BCL2L1, TP53, BCL2, CASP8, HSPA4, DIABLO and CASP7. The experimental work concludes that bioactive components present in the ethanolic leaf extract of A.indicum shows good anti-inflammatory and anti-proliferative activity by inducing Apaf-1 through CASP9, CASP3, CYCS, BCL2L1, TP53, BCL2, CASP8, HSPA4, DIABLO and CASP7 network.