alexa Evaluation of Antimicrobial Activity of Synthesized Silver Nanoparticles using Phyllanthus amarus and Tinospora cordifolia Medicinal Plants
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

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Research Article

Evaluation of Antimicrobial Activity of Synthesized Silver Nanoparticles using Phyllanthus amarus and Tinospora cordifolia Medicinal Plants

Khushboo Singh, Manju Panghal, Sangeeta Kadyan and Jaya Parkash Yadav*

Department of Genetics, M.D. University, Rohtak-124001, Haryana, India

*Corresponding Author:
Jaya Parkash Yadav
Department of Genetics
M.D. University
Rohtak-124001, Haryana, India
Tel: 91-9416474640
E-mail: [email protected]

Received Date: November 03, 2014; Accepted Date: December 03, 2014; Published Date: December 13, 2014

Citation: Singh K, Panghal M, Kadyan S, Yadav JP (2014) Evaluation of Antimicrobial Activity of Synthesized Silver Nanoparticles using Phyllanthus amarus and Tinospora cordifolia Medicinal Plants. J Nanomed Nanotechnol 5:250. doi:10.4172/2157-7439.1000250

Copyright: © 2014 Singh K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The main aim of this study is to evaluate the comparison of antimicrobial efficacy of silver nanoparticles synthesized from aqueous plant extracts of Phyllanthus amarus and Tinospora cordifolia. The synthesized silver nanoparticles were characterzed by UV-VIS spectroscopy, Fourier Transform Infra-Red spectroscopy, Transmission Electron Microscopy, Dynamic Light Scattering and zeta potential. The antimicrobial activity of synthesized silver nanoparticles was compared with their respective plant extracts by agar well diffussion method and minimum inhibitory concentration was also calculated. The zone of inhibition varied in range of 12 ± 1 to17 ± 0.58 mm with 100 μg/ml silver nanoparticles concentration, while acetonic, methanolic and aqueous extracts of respective plants does not showed any activity even at 1 mg/ml i.e. 10 times more than that of silver nanoparticles. MIC of silver nanoparticles was found to be in a range of 6.25-25 μg/ml against all tested microbes. The antimicrobial activity of synthesized silver nanoparticles was higher than that of the standard drug i.e. streptomycin (for bacteria) and ketoconazole (for fungus). The synthesized nanoparticles of P. amarus and T. cordifolia have shown good antimicrobial efficacy as compared to plant extracts and may prove to be better antimicrobial agent against wide range of microbes.

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