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Special Issue Article
Evaluation of Culture Media for Growth Characteristics of Alternaria solani, Causing Early Blight of Tomato
Somnath Koley1* and Shyama Sundar Mahapatra2
1Department of Plant Pathology, Orissa University of Agriculture and Technology, Orissa, India
2Associate Director of Research, RRTTS, Ranital, Orissa University of Agriculture and Technology, Orissa-756111, India
- *Corresponding Author:
- Somnath Koley
Department of Plant Pathology
Orissa University of Agriculture and Technology
Bhubaneswar-751003 (Orissa), India
Tel: +919474050094
E-mail: [email protected]
Received date: April 29, 2015 Accepted date: June 15, 2015 Published date: June 25, 2015
Citation:Koley S, Mahapatra SS (2015) Evaluation of Culture Media for Growth Characteristics of Alternaria solani, Causing Early Blight of Tomato. J Plant Pathol Microbiol S1: 005. doi: 10.4172/2157-7471.1000S1-005
Copyright: © 2015 Koley S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Early blight is most common and devastating disease in tomato plant caused by deuteromycotina fungi, Alternaria solani. This fungus grows well in potato dextrose agar and Richard’s broth medium in vitro. The growth of the fungi were tested under culture in twelve different liquid and solid media and compared with each other. Potato dextrose agar and oat meal agar among solid media and Richard’s broth and Sabouraud’s broth among liquid media appeared to be better than other media for growth of tomato early blight causing fungi. The growth characteristics such as color of colony and substrate, margin of colony, topography of mycelium along with the sporulation of the test fungus were studied on these solid media. Fungus sporulation was best in oat meal agar media. Maximum growth of the fungus was observed at 8 days after inoculation with continuous increasing growth in the potato dextrose broth (PDB) medium, although growth rate was decreasing after the 2 days of inoculation. This study will be helpful for further investigations on the physiology of the fungus and management of the disease. This investigation may be useful for taxonomic study of the fungus.
