Evaluation of Cytotoxicity of Food in Human Hepatoma HepG2 Cells: Comet Assay Coupled to the MTT AssayRana Zaidi* and Pinki Rani Rawat
Department of Biochemistry, Faculty of Science, Jamia Hamdard, New Delhi-110062, India
- *Corresponding Author:
- Rana Zaidi
Department of Biochemistry, Faculty of Science
Jamia Hamdard, New Delhi-110062, India
Tel: +091-11-26059688 ext. 5518
E-mail: [email protected]
Received date: May 29, 2012; Accepted date: June 22, 2012; Published date: June 27, 2012
Citation: Zaidi R, Rawat PR (2012) Evaluation of Cytotoxicity of Food in Human Hepatoma HepG2 Cells: Comet Assay Coupled to the MTT Assay. J Nutr Food Sci 2:149 doi: 10.4172/2155-9600.1000149
Copyright: © 2012 Zaidi R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The cooking of meat has been found to generate compounds that possess extreme mutagenicity in short term tests. Heterocyclic aromatic amines are potent bacterial and eukaryotic cell mutagens. In this study we employed two in vitro techniques, the MTT cell viability assay and the single cell gel electrophoretic assay to evaluate food genocytotoxicity in human hepatoma hepG2 cells in home cooked and commercially available food sources. Both representatitve assays confirm that PhIP (2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine) and MeIQx (2-amino-3,8 dimethylimidazo [4,5-f]quinoxaline) are potent DNA damaging agents in the selected cell line (HepG2). This study correlates the effects of exposure of food carcinogens to humans; we further propose such studies would lead to a better understanding of the risks involved for prevention of liver carcinomas.