alexa Evaluation of DAVIH-BLOT System for Simultaneous Diagnosis of HIV-1/2
ISSN 2155-6113

Journal of AIDS & Clinical Research
Open Access

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Research Article

Evaluation of DAVIH-BLOT System for Simultaneous Diagnosis of HIV-1/2

Otto Cruz Sui1*, Dervel Felipe Díaz Herrera2, Lucy Montano Tamayo3, Kenia Romero Martínez2, María Teresa Pérez Guevara4 and Eladio Silva Cabrera5

1Biologist, MSc degree in Biochemistry, Health Sciences PhD, Senior Researcher, AIDS Research Laboratory, Cuba

2Biochemistry, MSc degree in Biochemistry, Adjunct Researcher, AIDS Research Laboratory, Cuba

3Chemical Analysis Technician, AIDS Research Laboratory, Cuba

4Biochemistry, MSc degree in Biochemistry, Associate Researcher, Jamaica

5Specialist II degree in Microbiology, Veterinary Sciences PhD, Senior Researcher, AIDS Research Laboratory, Cuba

*Corresponding Author:
Otto Cruz Sui
Health Sciences, Senior Researcher
AIDS Research laboratory (lisida)
laboratorios davih
Tapaste Road and National Highway, Cuba
Tel: 047-848700, 047-848727
E-mail: [email protected]; [email protected]

Received Date: June 08, 2011; Accepted Date: September 21, 2012; Published Date: September 24, 2012

Citation: Sui OC, Herrera DFD, Tamayo LM, Martínez KR, Guevara MTP, et al. (2013) Evaluation of DAVIH-BLOT System for Simultaneous Diagnosis of HIV-1/2. J AIDS Clinic Res S1:020. doi:10.4172/2155-6113.S1-020

Copyright: © 2013 Sui OC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Validation of performance characteristics of HIV diagnostic tests is one of the main demands of regulatory bodies. Evaluating of diagnostics tests prior to use avoid errors with a high economic and social cost. The aim of the work was to validate the DAVIH-BLOT modified system for simultaneous diagnosis of HIV-1/2. An evaluation with 166 samples of reference panels and a comparative study against a commercial kit of similar characteristics with an international reference panel of 25 samples was made. The new system expanded its diagnostic capacity with the incorporation of a gp36 synthetic peptide-protein conjugate, obtaining high results of sensitivity and specificity in the performance evaluation (99.18 and 76.74% respectively). In the comparative study with the analogue system the concordance was very good (kappa=1). The inclusion of the synthetic peptide and natural proteins in the same solid phase, constitute a technological novelty to this assay. The application of this test, allows a short cut of the diagnostic algorithm when are used screening tests for HIV-1 and 2 as in the Cuba’s diagnostic algorithm.

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