Evaluation of Diuretic Activity of Different Solvent Fractions of Methanol Extract of Carissa edulis Root Bark in RatsSelamu Kebamo1, Eyasu Makonnen2, Asfaw Debella3 and Bekesho Gelet3*
- *Corresponding Author:
- Bekesho Gelet
Directorate of Traditional and Modern Medicine Research
Ethiopian Public Health Institute, PO Box 1242
Addis Ababa, Ethiopia
E-mail: [email protected]
Received date: October 17, 2015; Accepted date: October 28, 2015; Published date: October 31, 2015
Citation: Kebamo S, Makonnen E, Debella A, Gelet B (2015) Evaluation of Diuretic Activity of Different Solvent Fractions of Methanol Extract of Carissa edulis Root Bark in Rats. Med chem S2:003. doi: 10.4172/2161-0444.1000003
Copyright: © 2015 Kebamo S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Carissa edulis is used traditionally for the treatment of HIV/AIDS symptoms, rheumatism, gonorrhea, syphilis, rabies, malaria, epilepsy, chronic joint pain, and as a diuretic.
Objective: The present study investigates the diuretic activity of different solvent fractions of 80% methanol Soxhlet extract of Carissa edulis root bark in normal wistar rats.
Materials and methods: The volumes of urine output and urinary electrolyte were the parameters determined by oral administration of single doses of different solvent fractions of 80% methanol Soxhlet extract of Carissa edulis root bark at three dose levels (50, 500 and 1000 mg/kg) in normal wistar rats.
Results: The petroleum ether and n-butanol fractions showed no significant effect on the urine output and urinary excretion of K+ and Cl- at all tested doses. Urinary excretion of Na+ was, however, affected by the petroleum ether fraction (p<0.002 at 1000 mg/kg) and n-butanol fraction (p<0.05 at 50 mg/kg; p<0.03 1000 mg/kg). The aqueous fraction significantly increased urine output in a dose dependent manner (p<0.005 at 50 mg/kg; p<0.001 at 500 and 1000 mg/kg). It also significantly increased urinary excretion of Na+ (p<0.006 at 500 mg/kg; p<0.001 at 1000 mg/kg) and Cl- (p<0.05 at 50 mg/kg; p<0.001 at 500 and 1000 mg/kg) in a dose dependent manner. Moreover, the aqueous fraction produced no acute toxicity at the assayed dose, which was also consistent with previous results from mice model.
Conclusion: These findings collectively indicate that aqueous fraction exhibited significant diuretic activity, providing evidence, at least in part, for its folkloric use.