Evaluation of Endometrial Endometrioid Carcinoma Grade Using Ultraviolet- Visible-Light Microscopic SpectroscopyHaruhiko Yoshioka1*, Hironori Kondo2, Kayo Horie1, Kiyotada Washiya1 and Jun Watanabe1
- *Corresponding Author:
- Haruhiko Yoshioka
Hirosaki University Graduate School of Health Sciences
66-1 Honchou. Hirosaki, Aomori 036-8564, Japan
Tel: +81 172 39 5972
E-mail: [email protected]
Received Date: October 22, 2015 Accepted Date: November 05, 2015 Published Date: November 07, 2015
Citation: Yoshioka H, Kondo H, Horie K, Washiya K, Watanabe J (2015) Evaluation of Endometrial Endometrioid Carcinoma Grade using Ultraviolet- Visible-Light Microscopic Spectroscopy. J Cytol Histol 6:381. doi:10.4172/2157-7099.1000381
Copyright: © 2015 Yoshioka H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: The objective of this study was to investigate the possibility of an objective index to evaluate the grades of endometrial endometrioid carcinomas by analyzing the spectral values of ultraviolet(UV) and visible(Vis) transmittances, and the degree of UV- absorbing materials influencing nuclear colors using ultraviolet- visible-light microscopic spectroscopy (UV-Vis MS).
Study design: The materials were histological preparations from 4 patients, each with proliferative-phase endometrium (PPE) and grade 1(G1) and grade 3 (G3) of endometrial endometrioid carcinomas. Both spectra were measured in the same 50 nuclei of each specimen.
Results: 1) In the ultraviolet spectrum, transmittance of 260, 280, 300, and 320 nm were decreased in G3 compared to those in G1. All of the ultraviolet absorbing materials increased in G3. 2) In the visible spectrum, 520 nm (purplish red), 540 nm (purple), and 560 nm (violet) in G3 were deeper than all colors in G1, and violet in G3 was lighter than purplish red and purple in G1. 3) The following 3 characteristics were clarified with regard to the relationship between UV-absorbing materials and the violet color based group. 1) In G3, the number of the significant canonical correlation coefficient between ultraviolet light-absorbing materials and the violet color based group was only at one. 2) Purplish red and purple of the nuclei in G3 was strongly influenced by ultraviolet lightabsorbing materials (260-320 nm). 3) Purple and violet in G1 was strongly influenced by light-absorbing materials at 300 nm and 320 nm.
Conclusion: It was indicated that UV-Vis MS analysis could be an objective index to evaluate the grade of endometrial endometrioid cancerby analyzing the spectral values of transmittances of ultraviolet and visible light, and the degree of ultraviolet light- absorbing materials influencing nuclear colors in the same nuclear region.