alexa Evaluation of Fibronectin-Binding Protein Ag85-B as Tar
ISSN: 2161-1068

Mycobacterial Diseases
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Research Article

Evaluation of Fibronectin-Binding Protein Ag85-B as Target for Serodiagnosis of Swine Mycobacteriosis in Living Animals

Amr M. Mohamed1,2*, Ghada A. Abou El-Ella1,2, Essam A. Nasr3 and Youssef A. Soliman4

1Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt

2Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Saudi Arabia

3Bacterial Diagnostic Product, Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt

4Central Laboratory for Quality Control of Veterinary Biologics, Abbassia, Cairo, Egypt

Corresponding Author:
Amr M. Mohamed
Department of Animal Medicine,
Faculty of Veterinary Medicine
Assiut University, Assiut, Egypt
E-mail:[email protected]

Received Date: March 28, 2012; Accepted Date: May 18, 2012; Published Date: May 21, 2012

Citation: Mohamed AM, El-Ella GAA, Nasr EA, Soliman YA (2012) Evaluation of Fibronectin-Binding Protein Ag85-B as Target for Serodiagnosis of Swine Mycobacteriosis in Living Animals. J Mycobac Dis 3:124. doi:10.4172/2161-1068.1000124

Copyright: © 2012 Mohamed AM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



The aim of the current study was to evaluate the fibronectin-binding protein Ag85-B as a potential antigen for ELISA-based serodiagnosis of swine mycobacteriosis in living pigs. The validity of Ag85-B, in comparison with tuberculin purified protein derivatives (PPD), for the serodiagnosis of swine mycobacteriosis was investigated in the current study. A total of 89 serum samples from previously confirmed Mycobacterium and non-Mycobacteriuminfected slaughtered pigs were used for the evaluation study. These included 66 serum samples from confirmed cases with swine mycobacteriosis and 23 samples from non-Mycobacterium infected cases. Obtained ELISA results revealed an overall accuracy index, sensitivity, specificity, positive predictive value, and negative predictive value of 87.6%, 87.9%, 86.9%, 95.1%, and 71.4%, respectively for Ag85-B as compared to 80.9%, 86.4%, 65.2%, 87.7%, and 62.5%, respectively for tuberculin PPD. In conclusion, the study revealed the reliability of Ag85-B as a potential candidate for ELISA-based serological assay that would provide for early and rapid diagnosis of swine mycobacterial infections in living animals.

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