alexa Evaluation of Fibronectin-Binding Protein Ag85-B as Target for Serodiagnosis of Swine Mycobacteriosis in Living Animals
ISSN: 2161-1068

Mycobacterial Diseases
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Evaluation of Fibronectin-Binding Protein Ag85-B as Target for Serodiagnosis of Swine Mycobacteriosis in Living Animals

Amr M. Mohamed1,2*, Ghada A. Abou El-Ella1,2, Essam A. Nasr3 and Youssef A. Soliman4

1Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt

2Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Saudi Arabia

3Bacterial Diagnostic Product, Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt

4Central Laboratory for Quality Control of Veterinary Biologics, Abbassia, Cairo, Egypt

Corresponding Author:
Amr M. Mohamed
Department of Animal Medicine,
Faculty of Veterinary Medicine
Assiut University, Assiut, Egypt
E-mail:[email protected]

Received Date: March 28, 2012; Accepted Date: May 18, 2012; Published Date: May 21, 2012

Citation: Mohamed AM, El-Ella GAA, Nasr EA, Soliman YA (2012) Evaluation of Fibronectin-Binding Protein Ag85-B as Target for Serodiagnosis of Swine Mycobacteriosis in Living Animals. J Mycobac Dis 3:124. doi:10.4172/2161-1068.1000124

Copyright: © 2012 Mohamed AM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The aim of the current study was to evaluate the fibronectin-binding protein Ag85-B as a potential antigen for ELISA-based serodiagnosis of swine mycobacteriosis in living pigs. The validity of Ag85-B, in comparison with tuberculin purified protein derivatives (PPD), for the serodiagnosis of swine mycobacteriosis was investigated in the current study. A total of 89 serum samples from previously confirmed Mycobacterium and non-Mycobacteriuminfected slaughtered pigs were used for the evaluation study. These included 66 serum samples from confirmed cases with swine mycobacteriosis and 23 samples from non-Mycobacterium infected cases. Obtained ELISA results revealed an overall accuracy index, sensitivity, specificity, positive predictive value, and negative predictive value of 87.6%, 87.9%, 86.9%, 95.1%, and 71.4%, respectively for Ag85-B as compared to 80.9%, 86.4%, 65.2%, 87.7%, and 62.5%, respectively for tuberculin PPD. In conclusion, the study revealed the reliability of Ag85-B as a potential candidate for ELISA-based serological assay that would provide for early and rapid diagnosis of swine mycobacterial infections in living animals.

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords