Evaluation of GenotypeÃÂ® MTBDRplus Assay for Rapid Detection of Resistance to Isoniazid and Rifampin in Mycobacterium tuberculosis Isolates Collected in Tunisia
Manel Marzouk*, Imen Ben Kahla, Mohamed Dhaou, Asma Ferjani, Naila Hannachi and Jalel Boukadida
Laboratory of Microbiology and Immunology, UR12SP34, University Hospital Farhat Hached, Sousse, Tunisia
- Corresponding Author:
- Manel Marzouk
Laboratory of Microbiology and Immunology-UR12SP34-University Hospital
Farhat Hached- 4000, Sousse, Tunisia
E-mail: [email protected]
Received Date: January 24, 2014; Accepted Date: February 19, 2014; Published Date: February 26, 2014
Citation: Marzouk M, Khala IB, Dhaou M, Ferjani A, Hannachi N, et al. (2014) Evaluation of Genotype® MTBDRplus Assay for Rapid Detection of Resistance to Isoniazid and Rifampin in Mycobacterium tuberculosis Isolates Collected in Tunisia. J Mycobac Dis 4:143. doi:10.4172/2161-1068.1000143
Copyright: © 2014 Marzouk M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
TB diagnosis and resistance detection remain to suffer from complex techniques and long time taking. The rapid identification of drug-resistant strains of Mycobacterium tuberculosis is crucial for the timely initiation of appropriate antituberculosis therapy. Over the years, several new technologies have been proposed to accelerate and simplify the detection of resistant M. tuberculosis. In this context, we evaluated the Genotype MTBDRplus to detect resistance to isoniazid and rifampin in positive M. tuberculosis cultures. The performance of the Genotype MTBDRplus assay was compared with that of the phenotypic drug susceptibility tests, a gold standard culture based method. The Genotype MTBDRplus assay was quicker and more cost-effective for the detection of rifampin and isoniazid resistance, with a slightly lower detection of rifampin-resistant strains in our setting.