alexa Evaluation of Phytochemical, Chemical Composition, Antioxidant and Antimicrobial Screening Parameters of Rhamnus prinoides (Gesho) Available in the Market of Mekelle, Tigray, Ethiopia
ISSN: 2329-6836

Natural Products Chemistry & Research
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Research Article

Evaluation of Phytochemical, Chemical Composition, Antioxidant and Antimicrobial Screening Parameters of Rhamnus prinoides (Gesho) Available in the Market of Mekelle, Tigray, Ethiopia

Teklit Gebregiorgis Amabye*
Department of Chemistry, College of Natural and Computational Science, Mekelle University, Mekelle, Tigray, Ethiopia
Corresponding Author : Teklit Gebregiorgis Amabye
Department of Chemistry
College of Natural and Computational Science
Mekelle University, Mekelle
Tigray, Ethiopia
Tel: +251945523645
E-mail: [email protected]
Received November 24, 2015; Accepted December 12, 2015; Published December 17, 2015
Citation: Amabye TG (2015) Evaluation of Phytochemical, Chemical Composition, Antioxidant and Antimicrobial Screening Parameters of Rhamnus prinoides (Gesho) Available in the Market of Mekelle, Tigray, Ethiopia. Nat Prod Chem Res 4:198. doi:10.4172/2329-6836.1000198
Copyright: © 2015 Amabye TG. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

The objective of this study was the evaluation of phytochemical, chemical composition, antioxidant and antimicrobial screening parameters in leaf of Rhamnus prinoides which are used to impart bitterness to local alcoholic beverages in Ethiopia and as traditional medicine in some African countries. The phytochemical screening of aqueous and methanol/water plant extracts was performed using standard phytochemical techniques for the determination of the presence of alkaloids, steroids, triterpines, tannins, flavanoids, flavones, phenols, glycosides, anthraquinones, proteins and resins. The nutritional value of samples were analyzed for their chemical composition (moisture, proteins, fat, carbohydrates and ash) using AOAC (Association of Analytical chemists procedures. The antimicrobial activities of the plant extracts against Neisseria gonorrhea, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Pseudomonas aeroginosa, and Streptococcus fecalis using broth dilution technique were determined. These were compared with those of benzyl penicillin, oxytetracycline and streptomycin as positive controls in the experiment the extract of Rhamnus prinoides was positive for tannins, triterpenoids, cardiac glycosides, phenolics compounds, saponins and resins. Alkaloids were present in the methanolic/water extract only. The methanolic/water extract of Rhamnus prinoides also shows a broad spectrum of activity against all microorganisms tested except for Neisseria gonorrheae. This shows it has a high content of protein and fiber and is low in fat. The qualities demonstrate that it is this good alternative to other food stuffs and these results serve as the basis of further scientific study into various ways of enhancing the livelihood of particular areas of northern Mekelle through increased Rhamnus prinoides domestication as well as assessing the possible bioactivity of rhamnus against certain human diseases.

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