Evaluation of Pre-Clinical Efficacy to HPV16 L2E6E7 Vaccine and HPV16 E6E7 Adenovirus-5 Vector Vaccine with Different Dosages and Prime- Booster Regiments in Mouse Model
- *Corresponding Author:
- Zhuang Fangcheng
Institute of Viral Diseases
Zhejiang Academy of Medical Sciences
Hangzhou 310013, China
Tel: +86 571 88861601
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E-mail: [email protected]
Received date: June 13, 2013; Accepted date: June 24, 2013; Published date: June 28, 2013
Citation: Fang-Cheng Z, Gang C, Jie W, Su-feng J, Yun-shui J, Men G, et al. (2013) Evaluation of Pre-Clinical Efficacy to HPV16 L2E6E7 Vaccine and HPV16 E6E7 Adenovirus-5 Vector Vaccine with Different Dosages and Prime-Booster Regiments in Mouse Model. J Vaccines Vaccin 4:189. doi: 10.4172/2157-7560.1000189
Copyright: © 2013 Fang-Cheng Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Purpose: This study evaluates the dose-response and immunization procedure in mouse model and the efficacy of prime-booster regimens with HPV16 L2E6E7 vaccine and HPV16 E6E7 Ad5 vector vaccine. Methods: Experimental animals were C57 BL/6 mice. Each group included 10 or 20 C57 BL/6 mice. The tumor model used TC-1 tumor cells. The HPV16 L2E6E7 vaccine groups were treated using the following dosage: 15 μg/ml, 30 μg/ml, 60 μg/ml, 120 μg/ml, 240 μg/ml, and then 120 μg/ml was used for the following regimens: 0-7 days, 0-15 days, 0-7-15 days. The HPV16 E6E7 Adenovirus-5 vector vaccine groups were treated using the following dosage: 3.00×106 IU/ml, 3.00×107 IU/ml, 3.00×108 IU/ml, 3.00×109 IU/ml, and then 3.00×107 IU/ml was used for the following regimens: 0-7 days, 0-15 days, 0-7-15 days, and control group. Prime-booster combined regimens with HPV L2E6E7 vaccine (P, 120 μg/ml) and HPV16 E6E7 ad5 vector vaccine (V, 3.00×107 IU/ml) were set as follows: 0P-7P days, 0P-7V days, and 0P-7P-15V days, 0P-7V-15V days, and 0P-7P-15V-21V days. Results: Upon challenge with 104 TC-1 tumor cells, mice developed palpable, rapidly growing tumors within 7–14 days. These tumors became lethal to the mice within 21–28 days. HPV16 L2E6E7 vaccine (120 μg/ml, 0-7- 15 day’s procedure) protective efficacy was 85% and the HPV16 E6E7 Ad5 vector vaccine (3.00×107 IU/ml, 0 day procedure) that was 80%. Prime-booster regimens showed a protective efficacy of 80–90% for the 0P-7V days and 0P-7V-15V day’s schedules. Conclusion: HPV16 L2E6E7 vaccine and HPV16 E6E7 Ad5 vector vaccine are proved the candidate vaccine for therapeutic intervention against HPV16-induced tumor.