alexa Evaluation of Pre-Clinical Efficacy to HPV16 L2E6E7 Vac
ISSN: 2157-7560

Journal of Vaccines & Vaccination
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Evaluation of Pre-Clinical Efficacy to HPV16 L2E6E7 Vaccine and HPV16 E6E7 Adenovirus-5 Vector Vaccine with Different Dosages and Prime- Booster Regiments in Mouse Model

Zhuang Fang-Cheng1,2*, Chen Gang1, Wu Jie2, Jin Su-feng2, Jiang Yun-shui1, Gao Men1, Li Jian-buo3, Zhao Li4, Mao Zian3 and Tian Houwen4

1Institute of Viral Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, P.R. China

2Zhejiang Key Laboratory of Bio-medical Vaccine R and D, Hangzhou, P.R. China

3Zhejiang Pukang Biotechnology Co., Ltd., Hangzhou, P.R. China

4Institute of Viral Disease Prevention and Control, National Centre for Diseases Prevention & Control, Beijing, P.R. China

*Corresponding Author:
Zhuang Fangcheng
Institute of Viral Diseases
Zhejiang Academy of Medical Sciences
Hangzhou 310013, China
Tel: +86 571 88861601
Fax: +86 571 89890270
E-mail: [email protected]

Received date: June 13, 2013; Accepted date: June 24, 2013; Published date: June 28, 2013

Citation: Fang-Cheng Z, Gang C, Jie W, Su-feng J, Yun-shui J, Men G, et al. (2013) Evaluation of Pre-Clinical Efficacy to HPV16 L2E6E7 Vaccine and HPV16 E6E7 Adenovirus-5 Vector Vaccine with Different Dosages and Prime-Booster Regiments in Mouse Model. J Vaccines Vaccin 4:189. doi: 10.4172/2157-7560.1000189

Copyright: © 2013 Fang-Cheng Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Purpose: This study evaluates the dose-response and immunization procedure in mouse model and the efficacy of prime-booster regimens with HPV16 L2E6E7 vaccine and HPV16 E6E7 Ad5 vector vaccine. Methods: Experimental animals were C57 BL/6 mice. Each group included 10 or 20 C57 BL/6 mice. The tumor model used TC-1 tumor cells. The HPV16 L2E6E7 vaccine groups were treated using the following dosage: 15 μg/ml, 30 μg/ml, 60 μg/ml, 120 μg/ml, 240 μg/ml, and then 120 μg/ml was used for the following regimens: 0-7 days, 0-15 days, 0-7-15 days. The HPV16 E6E7 Adenovirus-5 vector vaccine groups were treated using the following dosage: 3.00×106 IU/ml, 3.00×107 IU/ml, 3.00×108 IU/ml, 3.00×109 IU/ml, and then 3.00×107 IU/ml was used for the following regimens: 0-7 days, 0-15 days, 0-7-15 days, and control group. Prime-booster combined regimens with HPV L2E6E7 vaccine (P, 120 μg/ml) and HPV16 E6E7 ad5 vector vaccine (V, 3.00×107 IU/ml) were set as follows: 0P-7P days, 0P-7V days, and 0P-7P-15V days, 0P-7V-15V days, and 0P-7P-15V-21V days. Results: Upon challenge with 104 TC-1 tumor cells, mice developed palpable, rapidly growing tumors within 7–14 days. These tumors became lethal to the mice within 21–28 days. HPV16 L2E6E7 vaccine (120 μg/ml, 0-7- 15 day’s procedure) protective efficacy was 85% and the HPV16 E6E7 Ad5 vector vaccine (3.00×107 IU/ml, 0 day procedure) that was 80%. Prime-booster regimens showed a protective efficacy of 80–90% for the 0P-7V days and 0P-7V-15V day’s schedules. Conclusion: HPV16 L2E6E7 vaccine and HPV16 E6E7 Ad5 vector vaccine are proved the candidate vaccine for therapeutic intervention against HPV16-induced tumor.

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords