Evaluation of Serum Antioxidants during Adjuvant Chemotherapy of Breast Cancer- A Prospective Observational Study
Kadam Charushila Y* and Abhang Subodhini A
Department of Biochemistry, Byramjee Jeejeebhoy government medical college, Pune, India
- *Corresponding Author:
- Kadam Charushila Y
Department of Biochemistry
Byramjee Jeejeebhoy government medical college
E-mail: [email protected]
Received Date: January 27, 2015; Accepted Date: April 21, 2015; Published Date: April 23, 2015
Citation: Charushila YK and Subodhini AA (2015) Evaluation of Serum Antioxidants during Adjuvant Chemotherapy of Breast Cancer- A Prospective Observational Study. Biochem Anal Biochem 4:171. doi: 10.4172/2161-1009.1000171
Copyright: © 2015 Kadam CY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: The enzymatic and non-enzymatic antioxidant defense systems of the cell prevent oxidant mediated damage to different biomolecules by neutralizing free radicals generated during anticancer drug metabolism. Reduced glutathione (GSH) and glutathione dependent enzymes such as glutathione peroxidase (GPx) and glutathione-s-transferase (GST) are the key determinants of cellular response to oxidative stress. The objective of this study was to evaluate changes in serum levels of these antioxidants after first cycle of adjuvant chemotherapy in breast cancer patients.
Material and Methods: Histopathologically proven 60 breast cancer patients were included in the present study. The blood samples were taken after surgery before chemotherapy and after 3 weeks of administration of first cycle of chemotherapy. 30 healthy female controls were selected for comparison. Serum GSH and GST were estimated by spectrophotometric methods and serum GPx was estimated by ELISA.
Results: The serum levels of GSH, GST and GPx were significantly decreased (P<0.0001) after first cycle of adjuvant chemotherapy in breast cancer patients as compare to levels before chemotherapy. A significant positive correlation was found between serum levels of GSH and GST as well as GSH and GPx after first chemotherapy cycle.
Conclusion: Our data suggests that administration of chemotherapeutic drugs causes depletion of reduced glutathione and glutathione-dependent enzymatic antioxidants such as GST and GPx, which may be due to higher oxidative stress after chemotherapy.