Evaluation of the Anti-asthmatic and Antitussive Effects of Aqueous Leaf Extract of Ocimum gratissimum in Rodents
|Ozolua RI1*, Umuso DI1, Uwaya DO2, Modugu AA1, Oghuvwu SO3 and Olomu JM4|
|1Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Benin, Benin City, 300001, Nigeria|
|2Department of Science Laboratory Technology, Faculty of Life Sciences, University of Benin, Benin City 300001, Nigeria|
|3Department of Internal Medicine, University of Benin Teaching Hospital, PMB 1111, Benin City, Nigeria|
|4Department of Animal Science, Faculty of Agriculture, University of Benin, Benin City 300001, Nigeria|
|*Corresponding Author :||Ozolua RI
Department of Pharmacology and Toxicology
Faculty of Pharmacy, University of Benin
Benin City, 300001, Nigeria
E-mail: [email protected] uniben.edu/[email protected] yahoo.co.uk
|Received: January 02, 2016; Accepted: February 03, 2016; Published: February 05, 2016|
|Citation: Ozolua RI, Umuso DI, Uwaya DO, Modugu AA, Oghuvwu SO, et al. (2016) Evaluation of the Anti-asthmatic and Antitussive Effects of Aqueous Leaf Extract of Ocimum gratissimum in Rodents. Med Aromat Plants 5:235. doi:10.4172/2167-0412.1000235|
|Copyright: © 2016 Ozolua RI, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Background: Ocimum gratissimum Linn (Lamiaceae) is an aromatic plant popular for it culinary uses. The aqueous leaf extract is used in ethnomedicine for the treatment of various diseases including asthma and cough. The purpose of this study was to evaluate the anti-asthmatic, antitussive and muco-suppressant effects of the aqueous leaf extract (OGE) in rodent species.
Methods: Ovalbumin-sensitized guinea pigs were exposed to 0.2% histamine aerosol in a glass chamber. Latency to preconvulsive dyspnea (PCD), tracheal fluid volume and viscosity were measured. For the antitussive screening, guinea pigs were exposed to 7.5% citric acid aerosol in a glass chamber and the bouts of cough pre and post-acute exposure were recorded. Mucus expectoration was estimated in mice after seven-day treatment with OGE.
Results: Latency to preconvulsive dyspnea was not significantly increased by doses of 100, 200 and 400 mg/ kg of OGE when compared with distilled water-treated sensitized guinea pigs. Tracheal fluid volume but not viscosity was significantly (p<0.0001) reduced by all the doses of OGE when compared with distilled water-treated sensitized guinea pigs. Also, the doses of OGE significantly reduced (p<0.0001) the number of cough bouts when compared with distilled water control. All the doses of extract significantly (p<0.003) reduced phenol red dye expectoration from mice tracheae.
Conclusion: The aqueous leaf extract of O. gratissimum does not protect against acute bronchospasms but possesses antitussive and muco-suppressant effects that may be helpful in asthmatics. Central mechanism may be contributory to the antitussive effect. These results lend credence to its ethnomedicinal use in the treatment of these diseases.