alexa Evaluation of the Effects of Ethanol on Monolayer and 3D Models of Human Oral Mucosa | OMICS International | Abstract
ISSN: 2161-0525

Journal of Environmental & Analytical Toxicology
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Research Article

Evaluation of the Effects of Ethanol on Monolayer and 3D Models of Human Oral Mucosa

Keyvan Moharamzadeh*, Kirsty L Franklin, Louise E Smith, Ian M Brook and Richard van Noort
School of Clinical Dentistry, University of Sheffield, United Kingdom
Corresponding Author : Dr. Keyvan Moharamzadeh
School of Clinical Dentistry, University of Sheffield
Claremont Crescent, Sheffield, S10 2TA, United Kingdom
Tel: +44 114 2717931
Fax: +44 114 2265484
E-mail: [email protected]
Received February 03, 2015; Accepted February 15, 2015; Published February 19, 2015
Citation: Moharamzadeh K, Franklin KL, Smith LE, Brook IM. van Noort R (2015) Evaluation of the Effects of Ethanol on Monolayer and 3D Models of Human Oral Mucosa. J Environ Anal Toxicol 5:275. doi: 10.4172/2161-0525.1000275
Copyright: © 2015 Moharamzadeh K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The aim of this study was to examine and compare the effects of different ethanol-containing solutions on a 3D oral mucosal model (OMM) with those of fresh oral mucosal biopsies and monolayer keratinocyte cultures. 3D OMMs were engineered by the air/liquid interface culture technique using human oral fibroblasts and keratinocytes. Fresh tissue biopsies were also obtained from patients and divided into small pieces. OMMs, fresh biopsies, and monolayer cultures were exposed to serial dilutions of ethanol in PBS (5% to 50%) for 30 secondss and 60 secondss. Biological response was evaluated using basic histology and the Alamar Blue tissue viability assay. Histological examination showed that the effects of ethanol on the OMMs resembled the fresh tissue biopsies. The 3D model was also able to detect the concentration-dependent and time-dependent effects of ethanol. The TC50 values for the monolayer cultures were significantly lower than those for the 3D models. It can be concluded that ethanol in high concentrations significantly affects the oral mucosal model’s viability and histological appearance. The response of the OMM to ethanol exposure was similar to the response of the fresh clinical biopsies. Monolayer keratinocytes are more sensitive to ethanol than 3D models or biopsies.

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