Evaluation of the Expression of c-MYC Protein during Liver Fibrosis Progression in Chronic Hepatitis Developed by Hepatitis C Virus Infected PatientsTerezinha Morato Bastos de Almeida1, Regina Maria Cubero Leitão2, Flair José Carrilho3, Ana Maria Gonçalves da Silva3 and Shigueko Sonohara1*
- *Corresponding Author:
- Shigueko Sonohara
Disciplina de Oncologia
Depto. de Radiologia e Oncologia
Faculdade de Medicina, Universidade de São Paulo
São Paulo, Brasil, Av. Dr. Arnaldo
455, 4o. Andar, CEP 01246-903, Brasil
E-mail: [email protected]
Received date: January 10, 2014; Accepted date: July 29, 2014; Published date:July 31, 2014
Citation: de Almeida TMB, Leitao RMC, Carrilho FJ, da Silva AMG, Sonohara S (2014) Evaluation of the Expression of c-MYC Protein during Liver Fibrosis Progression in Chronic Hepatitis Developed by Hepatitis C Virus Infected Patients. J Carcinog Mutagen 5:182. doi: 10.4172/2157-2518.1000182
Copyright: © 2014 de Almeida, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data on the onset and progression of fibrosis related to c-MYC protein expression in chronic hepatitis by C virus are scarce. In this work we evaluated by immunohistochemical method the expression of c-MYC protein in the liver parenchyma without fibrosis, and during its progression. Seventy-eight liver samples from Hepatitis C virus infected patients with different degrees of fibrosis (F0 to F4) were studied. Eleven healthy liver samples were included. All samples from chronic hepatitis were stained by hematoxylin-eosin method, and classified by METAVIR scoring system. The expression of c-MYC protein was evaluated in 1000 hepatocytes from each parenchyma area by immunohistochemistry. Results showed that c-MYC expression in hepatocytes from fibrosis samples (F1-F4) was higher, and statistically significant (P<0.05) when compared with the control group. The same results were obtained when comparing F4 vs F0; F1; F2 and F3. In contrast, the comparisons between F0 vs control; F1 vs F2; F1 vs F3; and F2 vs F3 did not present significant differences (P>0.05). In conclusion, our study showed that c-MYC protein is present in the initial degrees of fibrosis, but over-expressed in F4. This fact suggests that alteration of c-MYC expression may induce genetic instability, deregulating the cell cycle and/or apoptosis, which might contribute to progression of fibrosis and ultimately predisposing to the development of hepatocellular carcinoma.