Evaluation of the Protective Effect of Hibiscus sabdariffa L. Calyx (Malvaceae) Extract on Arsenic Induced Genotoxicity in Mice and Analysis of its Antioxidant PropertiesIlika Ghosh, Sonia Poddar and Anita Mukherjee*
Cell Biology and Genetic Toxicology Laboratory, Department of Botany, University of Calcutta.35, Ballygunge Circular Road, Kolkata 700019, India
- *Corresponding Author:
- Anita Mukherjee
Cell Biology and Genetic Toxicology Laboratory
Department of Botany, University of Calcutta.35
Ballygunge Circular Road, Kolkata 700019, India
E-mail: [email protected] gmail.com
Received Date: September 18, 2014; Accepted Date: October 28, 2014; Published Date: November 04, 2014
Citation: Ghosh I, Poddar S, Mukherjee A (2014) Evaluation of the Protective Effect of Hibiscus sabdariffa L. Calyx (Malvaceae) Extract on Arsenic Induced Genotoxicity in Mice and Analysis of its Antioxidant Properties. Biol Med (Aligarh) 6:218. doi:10.4172/0974-8369.1000218
Copyright: © 2014 Ghosh I, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The present study envisages the antigenotoxic property of Hibiscus sabdariffa L. (Roselle) calyx extracts that is presumably attributed to its antioxidant properties. The dried calyx extracts of Roselle were administered to male albino mice at doses of 50, 100, and 150 mg/kg body weight for 7 days followed by a single dose of interperitoneal (i.p.) injection of sodium arsenite (2.5 mg/kg body weight) and the extent of DNA damage was analysed by widely used Comet assay. The results revealed that the calyx extract of Roselle inhibited the DNA damage induced by sodium arsenite in a dose dependent manner. The presence of phytochemical constituents such as polyphenols and flavonoids were ascribed to the observed changes. The antioxidant efficacy was substantiated by applying Ferric Reducing Antioxidant Power (FRAP) and 2, 2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) assays.