Evaluation of Tumor Growth in Treatment of Murine Melanoma by Transdermal Infusion of Etoposide by RadiofrequencySalomao-Junior A1,2, D’Agostino LG1,2 , Luna ACL1,2, Menezes FC2,3, Viana DC4, Rodrigues CJ5 and Maria DA1,2*
- *Corresponding Author:
- Durvanei Augusto Maria
Faculty of Medicine
University of Sao Paulo, Sao Paulo, Brazil
Email: [email protected]
Received date: November 23, 2015 Accepted date: January 18, 2016 Published date: January 22, 2016
Citation: Salomao-Junior A, D’Agostino LG, Luna ACL, Menezes FC, Viana DC, et al. (2016) Evaluation of Tumor Growth in Treatment of Murine Melanoma by Transdermal Infusion of Etoposide by Radiofrequency. J Clin Exp Dermatol Res 7:325. doi: 10.4172/2155-9554.1000325
Copyright: © 2016 Salomao-Junior, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: In order to make such a substance cross the epidermal barrier, was utilized a method of transdermal drug delivery system (TDDS) combined with the chemotherapeutic etoposide in the treatment of dorsal melanoma B16F10 tumor on C57BL/6J mice.
Methods: The treatment groups were as follows: etoposide followed by radiofrequency (RF); RF followed by etoposide; etoposide and controls. The animals were treated with delivery interval of 72 hours, for 20 days and were analyzed the tumor growth; weight and hematological profile. The histological analysis and cell cycle by flow cytometry were performed after end of the treatment period.
Results: The tumors treated with RF followed by etoposide showed slower growth compared to the tumors treated with etoposide followed by RF, and found that treatment with radiofrequency considerably increased the dorsal tumor growth. In fact, the increase in tumor mass is because the radiofrequency cause an inflammatory response and stimulate collagen production by fibroblasts. Conclusions: The results showed that the treatment groups RF followed by etoposide showed a high rate sub- G1 phase cells, indicating better therapeutic efficacy. Therefore, it is important to clarify that the referred technologies are not as harmless at it has been reported.