alexa Evidence for a Constitutive Cutinase in Ungerminated Conidia of Erysiphe necator Schwein
ISSN: 2157-7099

Journal of Cytology & Histology
Open Access

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Research Article

Evidence for a Constitutive Cutinase in Ungerminated Conidia of Erysiphe necator Schwein

Sylvain Schnee, Erwan Rougeux, Roger Pezet, Olivier Viret and Katia Gindro*

Agroscope Changins-Wädenswil, P.O. Box 1012, Route de Duillier 50, CH-1260 Nyon, Switzerland

*Corresponding Author:
Katia Gindro
Agroscope Changins-Wädenswil
P.O. Box 1012, Route de Duillier 50
CH-1260 Nyon, Switzerland
Tel: +41 22 363 43 74
E-mail: [email protected]

Received Date: November 03, 2013; Accepted Date: November 20, 2013; Published Date: November 22, 2013

Citation: Schnee S, Rougeux E, Pezet R, Viret O, Gindro K (2013) Evidence for a Constitutive Cutinase in Ungerminated Conidia of Erysiphe necator Schwein. J Cytol Histol 4:197. doi:10.4172/2157-7099.1000197

Copyright: © 2013 Schnee S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Soluble proteins of ungerminated conidia of Erysiphe necator exhibited esterase and cutinase activities, and such hydrolytic activities were measured in the parietal protein fraction. However, histochemical localisation of esterase activity was detected upon further fungal development, as well in elongating germ tubes and in appressoria. The esterase activity was spectrophotometrically quantified using para-nitrophenylbutyrate as a substrate, and cutinase activity was determined using 3H-labelled cutin. Histochemical localisation was determined using indoxyl acetate as a substrate. Diisopropyl fluorophosphate was used as inhibitor of serine hydrolase. The role of a putative constitutive parietal cutinase in the ungerminated conidia of E. necator in adhesion to the host and differentiation of infective structures, as well as implications for successful penetration, are discussed.

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