alexa Expansion of Semi-Automatic Processed Human Adipose-Derived Stem Cells in Medium Supplemented with Autologous Serum and Antioxidants
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

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Research Article

Expansion of Semi-Automatic Processed Human Adipose-Derived Stem Cells in Medium Supplemented with Autologous Serum and Antioxidants

Li-Yi Sun1#, Dian-Kun Li2,3,8#, Pao-Jen Chen4#, Yu-Yu Joyce Ho1, Jon-Son Kuo1,2, Ching-Feng Cheng1,2,5, Kuei-Fang Lee6, Yao-Jen Chang7* and Cheng-Yoong Pang1,2*
1Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
2Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan
3Department of Hematology and Oncology, Taichung Tzu Chi General Hospital, Taichung, Taiwan
4Department of Obstetrics and Gynecology, Min-Sheng General Hospital, Taoyuan, Taiwan
5Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
6Center for Medical Genetics, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
7Department of Surgery, Taipei Tzu Chi General Hospital, Taipei, Taiwan
8Department of Medicine, College of Medicine, Tzu-Chi University, Hualien, Taiwan
#These authors contributed equally to this study
Corresponding Authors : Cheng-Yoong Pang
Institute of Medical Sciences, Tzu Chi University, No. 701
Sec. 3, Zhongyang Rd., Hualien City 970, Hualien, Taiwan
Tel: +886-3-8561825, ext. 5613
Fax: +886-3-8562019
E-mail: [email protected]
  Yao-Jen Chang
Department of Surgery, Buddhist Tzu Chi General Hospital Taipei Branch
No. 289, Jianguo Rd., Xindian Dist., New Taipei City 231, Taiwan
Tel: 886-2-66289779 ext. 5011
Fax: 886-2-6628-3316
E-mail: [email protected]
Received March 17, 2014; Accepted April 07, 2014; Published April 09, 2014
Citation: Sun LY, Li DK, Chen PJ, Ho YJ, Kuo JS, et al. (2014) Expansion of Semi-Automatic Processed Human Adipose-Derived Stem Cells in Medium Supplemented with Autologous Serum and Antioxidants. J Stem Cell Res Ther 4:193. doi:10.4172/2157-7633.1000193
Copyright: © 2014 Sun LY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Objective: Excised fat tissue (EFT) is an excellent source of adipose-derived stem cells (ADSCs) for autologous cell therapy. However, the current technique for excising fat tissues with conventional surgical scissors and using culture medium containing fetal bovine serum (FBS) may not comply with Good Tissue Practice (GTP) guidelines. We thus developed a semi-automated process complying with the abovementioned guideline to manufacture clinicalgrade ADSCs from small volumes of EFT. Methods: A semi-automated process was developed by using a disposable disperser tube operated on a Tube Disperser Workstation to obtain stromal vascular fraction (SVF) from EFT. A culture medium supplemented with human autologous serum (HAS) and mesenchymal stem cell culture adjuvant (MCA) consisted of basic fibroblast growth factor (bFGF/FGF-2) and antioxidants, was formulated to expand ADSCs from SVF in vitro. Results: The semi-automated process can markedly enhance cell yields and reduce the operation time for mincing tissues from an hour down to a few minutes. The growth of ADSCs was slower in the 10% HAS medium than that in medium supplemented with FBS, but their growth in the 10% HAS medium containing MCA (10% HAS+MCA) was superior to both media. The 10% HAS+MCA medium also promoted adipogenesis, osteogenesis, chondrogenesis and increased the expression of CD44, CD73 and the stemness genes in the ADSCs. Conclusion: Our novel semi-automated process provides an efficient isolation of SVF, and the 10% HAS+MCA medium accelerates the growth of ADSCs from small amounts of EFT without losing their differentiation ability.

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